We narrowed to 10,138 results for: SEC

1. SECURE BE4max(R33A) (pBM608)

   Plasmid

   #140251

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Sec61 beta pAcGFP-C1 (802)

   Plasmid

   #62008

   Purpose

   mammalian expression of GFP-Sec61 beta

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Sec61 beta (SEC61B Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pEGFP-Sec22b-P33-shR

   Plasmid

   #208359

   Purpose

   Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b-P33 mutant

   Depositor

   Paula Nunes-Hasler

   Article

   Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

   Insert

   Sec22b (Sec22b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   four silent mutations (shRNA resistance), 33 aa p…

   Promoter

   CMV

   Available since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Intersectin I SH3 A domain (human)

   Plasmid

   #47413

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain A (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain A

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pRS405 SEC66-VAC8-RFP

   Plasmid

   #166843

   Purpose

   Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8, SEC66 Budding Yeast)
   

   Use

   Tags

   RFP and SEC66 transmembrane domain

   Expression

   Yeast

   Mutation

   SEC66 transmembrane domain

   Promoter

   VAC8 promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Intersectin I SH3 B domain (human)

   Plasmid

   #47414

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain B (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain B

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

7. GFP-Intersectin Full Length

   Plasmid

   #47407

   Depositor

   Peter McPherson

   Insert

   Intersectin
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

8. GFP-Clavesin1 (pEGFP-C1-Sec14a)

   Plasmid

   #47421

   Depositor

   Peter McPherson

   Article

   Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

   Insert

   Clavesin1 (CLVS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. Intersectin I SH3 C domain (human)

   Plasmid

   #47415

   Depositor

   Peter McPherson

   Article

   Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

   Insert

   Intersectin SH3 domain C (ITSN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   only SH3 domain C

   Promoter

   tac

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Intersectin I SH3 D domain (human)

    Plasmid

    #47416

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain D (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain D

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

11. Intersectin I SH3 E domain (human)

    Plasmid

    #47417

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    ITSN1 intersectin 1 (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain E

    Promoter

    tac

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

12. FLAG-Sec31a S799A #12#903

    Plasmid

    #42117

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    Sec31a (SEC31A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    779A

    Promoter

    CMV

    Available since

    Feb. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

13. GFP-Clavesin2 (pEGFP-C1-Sec14b)

    Plasmid

    #47420

    Depositor

    Peter McPherson

    Article

    Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

    Insert

    Clavesin2 (CLVS2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

14. GFP-Sec31a 4SA #33#638

    Plasmid

    #42124

    Depositor

    Ayano Satoh

    Article

    Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

    Insert

    Sec31a (SEC31A Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S527A S799A S1163A T1165A

    Promoter

    CMV

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pRS413-TEF1pr-SEC61-FAPoptim

    Plasmid

    #221126

    Purpose

    FAP-tagged marker for the ER (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC61-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS415-TEF1pr-SEC63-FAPoptim

    Plasmid

    #221116

    Purpose

    FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    FAPoptim-SEC63
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS415-TEF1pr-SEC61-FAPoptim

    Plasmid

    #221115

    Purpose

    FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC61-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS413-TEF1pr-SEC7-FAPoptim

    Plasmid

    #221124

    Purpose

    FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC7-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227894

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SEC61B homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227903

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge