We narrowed to 13,934 results for: TIM;

1. miniTn7(Gm)PA1/04/03-eyfp-a

   Plasmid

   #111620

   Purpose

   eYFP Fluorescent tagging of bacteria at their Tn7-site

   Depositor

   Tim Tolker-Nielsen

   Article

   Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

   Insert

   PA1/04/03-eyfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBAD-yhjH

   Plasmid

   #111613

   Purpose

   Arabinose-inducible expression of the E. coli cyclic di-GMP phosphodiesterase gene yhjH

   Depositor

   Tim Tolker-Nielsen

   Article

   Christensen et al Infect Immun. 2013 Aug;81(8):2705-13. do

   Insert

   yhjH
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A52V

   Promoter

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. miniTn7(Gm)PA1/04/03-ecfp-a

   Plasmid

   #111619

   Purpose

   eCFP Fluorescent tagging of bacteria at their Tn7-site

   Depositor

   Tim Tolker-Nielsen

   Article

   Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

   Insert

   PA1/04/03-ecfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC37

   Plasmid

   #111618

   Purpose

   Fluorescent biosensor for gauging the activity of the PQS quorum sensing system in Pseudomonas aeruginosa

   Depositor

   Tim Tolker-Nielsen

   Article

   Yang et al Microbiology. 2007 May;153(Pt 5):1318-28

   Insert

   PpqsA-gfp(ASV)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONRPEX18Km-SceI-pheS

   Plasmid

   #111610

   Purpose

   Gateway compatible allelic exchange vector for Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Type

   Empty backbone

   Use

   Allelic exchange in burkholderia sp.

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONRPEX18Gm-SceI-pheS

   Plasmid

   #111609

   Purpose

   Gateway compatible allelic exchange vector for Burkholderia sp.

   Depositor

   Tim Tolker-Nielsen

   Article

   Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

   Type

   Empty backbone

   Use

   Allelic exchange in burkholderia sp.

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

   Plasmid

   #193942

   Purpose

   a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.

   Depositor

   Greg Delgoffe

   Article

   Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122

   Insert

   heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

   Plasmid

   #163991

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized

    Plasmid

    #231602

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized under CMV promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag_codon_optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pD649-HAsp-TIMD4-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156731

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TIMD4 (TIMD4 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CycK-GFP (1-270AA) in Cilantro (Codon optimized)

    Plasmid

    #169930

    Purpose

    Overexpression of 1-270AA CycK-GFP fusion

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Insert

    CCNK (CCNK Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCW-codon optimized catalytically inactive PHGDH

    Plasmid

    #154903

    Purpose

    Expresses codon optimized catalytically inactive PHGDH in mammalian cells

    Depositor

    Michael Pacold

    Article

    Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

    Insert

    Phosphoglycerate dehydrogenase (PHGDH Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Three mutations: D175N, R236K, H283A

    Promoter

    TRE

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTR-339 pUC19-tNGFR-P2A-TIM3 N

    Plasmid

    #186077

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    TIM3 (HAVCR2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZ005 (AacC2c1 human codon optimized protein)

    Plasmid

    #70171

    Purpose

    Expresses AacC2c1 human codon optimized protein

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    AacC2c1 protein
    

    Use

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV hSyn INTRON HaloTag-STIM1 WPRE

    Plasmid

    #236233

    Purpose

    AAV expression of mouse STIM1 internally tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    HaloTag-Stim1 (Stim1 Mouse)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV hSyn INTRON mScarlet-STIM2 WPRE

    Plasmid

    #236236

    Purpose

    AAV expression of human STIM2 internally tagged with mScarlet-I

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mScarletI-STIM2 (STIM2 Human)
    

    Use

    AAV

    Tags

    mScarletI in position 29

    Expression

    Mutation

    Signal peptide from STIM1 and residues 15-746 of …

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV hSyn INTRON mEmerald-STIM1 WPRE

    Plasmid

    #236234

    Purpose

    AAV expression of mouse STIM1 internally tagged with mEmerald

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    mEmerald-Stim1 (Stim1 Mouse)
    

    Use

    AAV

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV hSyn INTRON HaloTag-STIM2 WPRE

    Plasmid

    #236235

    Purpose

    AAV expression of human STIM2 internally tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    HaloTag-STIM2 (STIM2 Human)
    

    Use

    AAV

    Tags

    HaloTag in position 29

    Expression

    Mutation

    Signal peptide from STIM1 and residues 15-746 of …

    Promoter

    human Synapsin 1

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpCas9(RRTIMR)-P2A-EGFP (AHK149)

    Plasmid

    #223071

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RRTIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(RRTIMR)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(RRTIMR)=D1135R, S1136R, G1218T, E1219I, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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