We narrowed to 5,659 results for: donor

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AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97309

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Expression

Bacterial and Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_CBh

Plasmid

#241376

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with CBh promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-Donor_UbC

Plasmid

#241375

Purpose

Bxb1-GA donor plasmid for cloning of payloads <20 kb with UbC promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pDONOR 6colors

Plasmid

#229974

Purpose

bigMamAct assembled plasmid for 6colors cellular labelling

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin

Use

CRISPR

Expression

Mammalian

Mutation

See Depositor Comments

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDONOR-MARINA

Plasmid

#223328

Purpose

DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).

Depositor

Jonathan Arias

Article

Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

Inserts

MARINA
KIR2.1 channel Golgi-to-plasma membrane trafficking signal

Use

CRISPR and Synthetic Biology

Tags

mCherry and super-ecliptic pHluorin

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDonorTp-fugfp

Plasmid

#211790

Purpose

pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites

Depositor

Silvia Cardona

Article

Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7

Insert

fuGFP

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[GFP]

Plasmid

#213903

Purpose

Mariner transposon expressing mNeonGreen with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and mNeonGreen

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#173208

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N mScarlet-I-TOSI donor

Use

CRISPR

Expression

Mammalian

Promoter

hPGK1

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[RFP]

Plasmid

#213904

Purpose

Mariner transposon expressing mCherry with cam selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and mCherry

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_RB-TnV2_pJEx

Plasmid

#213908

Purpose

Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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LT1_75_Donor_Tag_RPL13A-mTurq2

Plasmid

#194415

Purpose

Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

mTurquoise2 CDS

Use

Synthetic Biology; Used as donor dna in genome in…

Expression

Yeast

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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DUX4_KBM pDonor221

Plasmid

#187802

Purpose

DUX4 with KBM (p. E414-L424 deletion) entry clone

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

DUX4-KBM (DUX4 Human)

Expression

Mammalian

Mutation

p. E414-L424 deletion

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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phABCA3-eGFP_CRISPR_Donor

Plasmid

#188540

Purpose

Homologous recombination donor plasmid for CRISPR/Cas9 targeting of GFP fusion protein to the stop codon of endogenous human ABCA3 gene locus

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

EGFP

Use

CRISPR; Donor for homologous recombination

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe2-WT

Plasmid

#85845

Purpose

Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 2 homology arms (SNCA Human)

Tags

-

Expression

Bacterial and Mammalian

Mutation

-

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1 SA-2A-puro-pA donor

Plasmid

#22075

Purpose

AAV zinc finger donor vector for genomic targeting

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-Puro

Use

Zfn donor

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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CCR5-donor-MCS

Plasmid

#192269

Purpose

Donor template for CRISPR Genome Editing

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

MCS within donor template

Use

CRISPR

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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[#GS5-RV] MitoTRACER-Donor

Plasmid

#233505

Purpose

Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Retroviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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