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Plasmid#124403PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.DepositorInsertGal4-TET1-878-911(T898A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationT898APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L900A(pL84)
Plasmid#124402PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.DepositorInsertGal4-TET1-878-911(L900A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL900APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Gal4-TET1-878-911-L897A(pL84)
Plasmid#124401PurposeGal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.DepositorInsertGal4-TET1-878-911(L897A mutation) (TET1 Human)
UseTagsgal4 dna binding domainExpressionMammalianMutationL897APromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
1264 pBS RSV Gal4 FKHR
Plasmid#10707DepositorInsertFKHR (FOXO1 Human)
UseTagsGal4 DBDExpressionMammalianMutation(the Gal4 DBD is aa 1-147)PromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
Gal4BD-CRY2(535)
Plasmid#75369PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
B4GALT1-Dox
Plasmid#124633PurposeDoxycycline-inducible B4GALT1 expression in mammalian cellsDepositorInsertsBeta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PC1-29-9-2_GAL4-VHL
Plasmid#200434PurposeExpresses the GAL4 DNA Binding domain linked to the VHL E3 Ubiquitin LigaseDepositorInsertGAL4-VHL
UseTagsGAL4, VHLExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMX-Gal4-TEAD4
Plasmid#33105DepositorInsertTEAD4 (TEAD4 Human)
UseTagsGal4ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GalT-mCherry
Plasmid#214271PurposeMammalian expression of GalT-mCherry (Golgi marker)DepositorInsertGalT(1-82)-mCherry (B4GALT1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAI-GAL4-CREB
Plasmid#46769PurposeExpression of GAL4 fusion to wild type CREBDepositorUseTagsGAL4ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b K294R
Plasmid#118359PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.DepositorInserthHSF4b (HSF4 Human)
UseTagsExpressionMammalianMutationK294RPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1-YFP
Plasmid#18685DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRS426 Gal-RNQ1-YFP
Plasmid#18686DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseTagsYFPExpressionYeastMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pTS2373-Tier1-PhCMV-Gal4
Plasmid#169581PurposeTier-1 vector encoding PhCMV-driven Gal4 DNA binding domain (PhCMV-Gal4-pA).DepositorInsertregulatory protein GAL4
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal-RNQ1 L94A
Plasmid#18691DepositorInsertRnq1 L94A
UseTagsExpressionYeastMutationLeucine 94 mutated to Alanine (L94A)PromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2(fs)-Gal11p(fs)
Plasmid#127483PurposeNegative control for the AVA-seq system. LGF2 (with one nucleotide insertion) (lambda CI associated) and Gal11p (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertLGF2 (frame shift)-Gal11p (frame shift) (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationOne nucleotide inserted between lambda CI and LGF…PromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2(fs)
Plasmid#127482PurposeNegative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interactionDepositorInsertGal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationLGF2 is frame shifted by the insert of one nucleo…PromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only