Addgene

Showing: 1 - 20 of 2413 results

Results per page:

Sort results by:

psiTE(1)-Gal4DBD

Plasmid

#220453

Purpose

Split intein version of Trojan-Gal4DBD. Create Gal4DBD hemi-driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4DBD-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
psiTE(1)-Gal4

Plasmid

#220450

Purpose

Split intein version of Trojan-Gal4. Create Gal4 driver lines from MiMIC lines, in which MiMIC cassette was inserted into Phase 1 coding intron.

Depositor

Benjamin White

Article

Diao et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

Insert

CfaN-Gal4-CfaC

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-sgGAL4-1

Plasmid

#46915

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-1
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS_KS_attB2_SA(1)_T2AGAL4

Plasmid

#125212

Purpose

Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4-pcVP16-1

Plasmid

#231570

Purpose

Expresses the single component, light-off regulated pcVP16-1 transcriptional regulator

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Gal4-pcVP16-1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL4(1-147)

Plasmid

#195029

Purpose

The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4 (1-147)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGal4BD 1-147

Plasmid

#165819

Purpose

1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domain

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Gal4BD 1-147

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL-AR-1-503

Plasmid

#89093

Purpose

mammalian expression of human androgen receptor N-terminal 1-503 amino acids linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Langley et al J Biol Chem. 1995 Dec 15;270(50):29983-9

Insert

hAR aa 1-503 (AR Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only
GAL4-Creb5 (1- 508)

Plasmid

#195030

Purpose

GAL4-Creb5 fusion construct (full length, 508aa)

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (full length, 508aa) (CREB5 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-sfGFP(1-157)

Plasmid

#220743

Purpose

GAL4-sfGFP(1-157) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(1-157)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL4-Creb5 (1- 128)

Plasmid

#195031

Purpose

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa)

Depositor

Cheng-Hai Zhang, Andrew Lassar

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4-Creb5 fusion construct (N-terminus of Creb5,128aa) (CREB5 Bovine)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCA/GAL4(1-93).ER.VP16

Plasmid

#108216

Purpose

Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

Depositor

Didier Picard

Article

Louvion et al Gene 1993 Sep 6;131(1):129-34.

Insert

Gal4 DBD-ERalpha HBD-VP16

Use

Tags

Expression

Yeast

Mutation

ERalpha HBD has G400V mutation

Promoter

ADH1

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4 PGC1 alpha 1-550

Plasmid

#8894

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Tags

Gal4 DBD

Expression

Mammalian

Mutation

amino acids 1-550 of PGC-1 alpha

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCEFL GAL4dbd-KLF4 1-329

Plasmid

#140146

Purpose

GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 Kruppel factor (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 330 to 479

Promoter

EF1a

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Gal4 PGC1 alpha 1-350

Plasmid

#8893

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Tags

Gal4 DBD

Expression

Mammalian

Mutation

amino acids 1-350 of PGC-1 alpha

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pCEFL GAL4dbd-KLF4 1-156

Plasmid

#140147

Purpose

GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 1-156 (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 157 to 479

Promoter

EF1a

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA GAL4 CREB 1-341

Plasmid

#22972

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB

Use

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-501aa-YFP

Plasmid

#29601

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-501aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-501

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-373aa-YFP

Plasmid

#29598

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-373

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-170aa-YFP

Plasmid

#29596

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-170aa (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-170

Promoter

Available since

April 29, 2011

Availability

Academic Institutions and Nonprofits only