Addgene

We narrowed to 346 results for: pET3

Showing: 281 - 300 of 346 results

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POC1470

Plasmid

#213758

Purpose

BsaI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

Truncated non-switchable methylase M2.BsaI

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDK134

Plasmid

#134187

Purpose

Expression of C. elegans KNL1

Depositor

Iain Cheeseman

Article

Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

Insert

KNL1 aa 69 - 479 (knl-1 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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rk1s

Plasmid

#117424

Purpose

Expression of ARF1

Depositor

Richard Kahn

Article

Kahn et al J Biol Chem. 1991 Feb 5;266(4):2606-14.

Insert

ARF1 (ARF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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rk3s

Plasmid

#117426

Purpose

Expression of ARF4

Depositor

Richard Kahn

Article

Kahn et al J Biol Chem. 1991 Feb 5;266(4):2606-14.

Insert

ARF4 (ARF4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET-Gm1

Plasmid

#53528

Purpose

Expresses a plant arginyl-tRNA synthetase in E.coli

Depositor

Gabor Igloi

Article

Igloi et al FEBS J. 2009 Mar;276(5):1307-18. doi: 10

Insert

arginyl-tRNA synthetase

Use

Tags

His Tag and Thioredoxin

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 31, 2014

Availability

Academic Institutions and Nonprofits only

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T7-RelA(KR)

Plasmid

#23251

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K218/221R, K310R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
His-PIMT

Plasmid

#34852

Depositor

Bruce Downie

Article

Dinkins et al Plant J. 2008 Jul;55(1):1-13.

Insert

PIMT (PCMT1 Human)

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pCT310

Plasmid

#111140

Purpose

Bacterial expression of 6His-dCas9-3XFlag

Depositor

Robert Tjian

Article

Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

Insert

dCas9

Use

Tags

3X Flag and 6X His

Expression

Bacterial

Mutation

D10A - H840A

Promoter

T7

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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T7-RelA(K310R)

Plasmid

#23250

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K310R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pDC158

Plasmid

#115793

Purpose

Expresses Rat SNAP25

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

SNAP25 (Snap25 Rat)

Use

Tags

hexa histidine

Expression

Bacterial

Mutation

c84s, c85s, c90s, c92s

Promoter

T7

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDC191

Plasmid

#115792

Purpose

Expresses Rat synaptobrevin 2

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

Synaptobrevin2 (Vamp2 Rat)

Use

Tags

hexa histidine

Expression

Bacterial

Mutation

S28C and C103S (please see comments below)

Promoter

T7

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRham-CALM1

Plasmid

#182500

Purpose

Bacterial expression of the Homo sapiens calmodulin, CALM1

Depositor

Dan Mulvihill

Article

Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

Insert

CALM1 (CALM1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhamnose

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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T7-RelA(K221R)

Plasmid

#23248

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K221R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
pAR3040-spitz

Plasmid

#38341

Depositor

Norbert Perrimon

Article

Rutledge et al Genes Dev. 1992 Aug;6(8):1503-17.

Insert

spitz (spi Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
T7-RelA(K218R)

Plasmid

#23247

Depositor

Warner Greene

Article

Chen et al EMBO J. 2002 Dec 2. 21(23):6539-48.

Insert

NFkB RelA(K218R) (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

K218R

Promoter

Available since

March 15, 2010

Availability

Academic Institutions and Nonprofits only
Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pKM149

Plasmid

#134178

Purpose

Bacterial expression of his-CENP-C 510-end

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

his-CENP-C 510-end (CENPC Human)

Use

Tags

Expression

Bacterial

Mutation

aa 510-end

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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His8-TEV-PseH

Plasmid

#89725

Purpose

Expresses PseH from C. jejuni with an N-terminal 2xHis Gln 5x His feature, followed by the TEV cleavage site

Depositor

Barbara Imperiali

Article

Zamora et al Methods Enzymol. 2017;597:145-186. doi:

Insert

PseH (pseH Campylobacter jejuni)

Use

Tags

2xHis Gln 5xHis and TEV

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSY091

Plasmid

#110068

Purpose

Bacterial expression of L4

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L4

Use

Tags

Expression

Mutation

AT-swapped mutant

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSY075

Plasmid

#110067

Purpose

Bacterial expression of L2

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

L2

Use

Tags

Expression

Bacterial

Mutation

AT-swapped mutant

Promoter

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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