We narrowed to 3,461 results for: zebrafish
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Plasmid#132641PurposeExpresses ErCas12a and an sgRNA to target a region of interestDepositorInsertErCas12a
UseAAV and CRISPRTags3x HA and SV40 NLSExpressionMammalianMutationQ926R (Please see depositor comments) and BsaI si…PromoterCMV PromoterAvailable sinceApril 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMT-sox10-NLS-BirA-2A-mCherry_Ras
Plasmid#80063PurposeSox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequencesDepositorInsertBiotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
UseZebrafish biotaggingTags3x HAExpressionBacterialPromoterSox10 promoterAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq)
Plasmid#124432PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)DepositorInsertFlipGFP(Casp3 cleavage seq)
UseZebrafish (good expression)Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p2A-CreERT2-FRT-kan-FRT
Plasmid#108454PurposeThis plasmid can be used to recombine the mCherry-p2a-CreERT2-pA cassette (or only CreERT2-pA) into a BAC. Also to produce mCherry-p2A-CreERT2 mRNA using SP6.DepositorInsertmCherry-p2a-CreERT2
UseCre/LoxAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124431PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafishDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseZebrafish (if expression level is too high in zeb…Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT2-4xUAS:NICD-P2A-mRFP
Plasmid#127594PurposeZebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.DepositorInsertNICD (notch1a Zebrafish)
UseZebrafish expressionAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertgal4/VP16
UseSynthetic BiologyAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol2-10xUAS-Voltron
Plasmid#119039PurposeUAS-driven expresion of Voltron in zebrafishDepositorInsertVoltron
UseZebrafish expressionPromoterE1bAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-SV40
Plasmid#117807PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-eGFP-SV40
Plasmid#117813PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-RfxCas13d-NLS-HA
Plasmid#141321PurposePlasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)DepositorInsertRfx-Cas13d (NLS-RfxCas13d-NLS)
UseCRISPRTagsHA and NLSAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTol2-elavl3-Voltron-ST
Plasmid#119038PurposePan neuronal expression of soma-localized Voltron in zebrafishDepositorInsertVoltron-ST
UseZebrafish expressionPromoterelavl3Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGTag-TagRFP-CAAX-SV40
Plasmid#117809PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsCAAXAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
I-SceI pB 5UAS::ZfSyGCaMP2
Plasmid#26125DepositorAvailable sinceOct. 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
Antibody#180086-rAbPurposeAnti-Kv1.2 K+ channel (Human) recombinant mouse monoclonal antibodyDepositorRecommended applicationsImmunohistochemistry and Western BlotReactivityFrog, Human, Mouse, Rat, and ZebrafishSource speciesMouseIsotypeIgG2aTrial sizeAvailable to purchaseAvailable sinceMarch 28, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits
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Tol2-BetaActin-SecA5-YFP
Plasmid#105664PurposeUbiquitously expresses a secreted and fluorescently tagged Annexin5 for in-vivo apoptosis reporterDepositorAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-nfsB-CFP
Plasmid#86374Purposeexpression of nfsB-CFPDepositorInsertnitroreductase
UseZebrafishTagsECFPAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-MLS-KillerRed
Plasmid#115517PurposeMiddle entry plasmid containing membrane bound KillerRed fluorophoreDepositorInsertMLS-KillerRed
UseGateway CloningTagsMLSAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Plasmid kit#1000000087PurposeA plasmid collection, based on the Tol2kit, used to streamline the production of transgenic zebrafish for studying neurodegenerative disorders.DepositorApplicationCloning and Synthetic BiologyVector typeZebrafish ExpressionCloning typeMultiSite GatewayAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
sox10:mCherry
Plasmid#98695PurposeI-SceI meganuclease-mediated mCherry transgenesis in zebrafishDepositorInsertmCherry
UseZebrafish expressionAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only