We narrowed to 11,223 results for: NSI

1. pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138130

   Purpose

   Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. tdp43-EGFP construct6

   Plasmid

   #28199

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa1-314 only

   Promoter

   Available since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

3. FMRP shRNA

   Plasmid

   #206074

   Purpose

   expression of FMRP RNA for gene silencing in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Ferron et al Nat Commun. 2014 Apr 7;5:3628. doi: 10.1

   Insert

   FMRP (Fmr1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tdp43-EGFP construct7

   Plasmid

   #28200

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa1-274 only

   Promoter

   Available since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

5. tdp43-EGFP construct8

   Plasmid

   #28201

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa275-313 deleted

   Promoter

   Available since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pRS416 Gal Q331K YFP

   Plasmid

   #27450

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Glutamine 331 mutated to Lysine (Q331K)

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pLVX-NPC1(P691S)-FLAG

   Plasmid

   #164973

   Purpose

   Lentiviral vector for NPC1 expression in mammalian cells

   Depositor

   Roberto Zoncu

   Article

   Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

   Insert

   NPC1 (NPC1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   P691S

   Promoter

   EF1a

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mEGFP-HRas S17N

   Plasmid

   #18665

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   S17N mutation in H-Ras

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 AK3 Hyg

   Plasmid

   #37539

   Depositor

   David Sidransky

   Article

   Chang et al PLoS One. 2011;6(6):e20806. Epub 2011 Ju

   Insert

   AK3 (AK3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCA528 AMSH 1-178

    Plasmid

    #160050

    Purpose

    Expresses MIT domain (residues 1-178) of AMSH as a HIS-SUMO fusion protein. Internal ID: WISP18-22.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    AMSH residues 1-178 (STAMBP Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-178

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)

    Plasmid

    #191164

    Purpose

    Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP (additional cutsites)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-ATP6V1D-HAGST

    Plasmid

    #87932

    Purpose

    C-term HA-GST tagged overexpression

    Depositor

    David Sabatini

    Article

    Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

    Insert

    ATP6V1D (ATP6V1D Human)
    

    Use

    Tags

    HA-GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTRIPZ-EGFP:LIMD1

    Plasmid

    #108230

    Purpose

    Lentiviral vector for Tet-inducible LIMD1 expression

    Depositor

    Kenneth Irvine

    Article

    Ibar et al J Cell Sci. 2018 Feb 13. pii: jcs.214700

    Insert

    LIMD1 (LIMD1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Tet/ON Minimal CMV promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-shCTRL

    Plasmid

    #181875

    Purpose

    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    control shRNA with no known targets in the mouse genome
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-NPC1(F503/4A)-FLAG

    Plasmid

    #164974

    Purpose

    Lentiviral vector for NPC1 expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

    Insert

    NPC1 (NPC1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F503A and F504A

    Promoter

    EF1a

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-FLAG-Sestrin2

    Plasmid

    #72595

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN2 (SESN2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pAAV_18xTEAD_luc2

    Plasmid

    #173003

    Purpose

    firefly luciferase reporter vector with 18x clustered TEAD binding sites

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    18xTEAD-luc2
    

    Use

    AAV and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV5B+longBRD4

    Plasmid

    #157792

    Purpose

    Expression of long isoform of BRD4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    long BRD4 (BRD4 Human)
    

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-PPP1CB-miRFP703

    Plasmid

    #178524

    Purpose

    Expresses PP1c-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    PP1c-miRFP703 (PPP1CB Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC1 Grx1-roCherry-mito

    Plasmid

    #141069

    Purpose

    Fluorescent biosensor for 2GSH/GSSG ratio with mitochondrial localization

    Depositor

    Dmitry Bilan

    Article

    Shokhina et al Redox Biol. 2019 Feb;21:101071. doi: 10.

    Insert

    Grx1-roCherry
    

    Use

    Tags

    dMTS

    Expression

    Mammalian

    Mutation

    Changed Alanine 150 to Cysteine, inserted Threoni…

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR-JNKKTRmRuby2

    Plasmid

    #59148

    Purpose

    ORF encoding JNK KTR mRuby2 flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGPTVII-SED1

    Plasmid

    #232615

    Purpose

    SED1 biosensor for expression in Arabidopsis thaliana

    Depositor

    Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 Biosensor
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pY001 (pFnCpf1_full)

    Plasmid

    #69973

    Purpose

    Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-RAB-EKARev-NLS

    Plasmid

    #118476

    Purpose

    ddRFP-based single-color ERK biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev-NLS
    

    Use

    Tags

    6xHIS, Nuclear localization signal (NLS), T7 tag …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX-4T1-USP11

    Plasmid

    #78849

    Purpose

    To purify GST-tagged recombinant USP11 protein

    Depositor

    Jaewhan Song

    Article

    Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

    Insert

    USP11 (USP11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

26. LentiCRISPRv2 NT sgRNA3

    Plasmid

    #164929

    Purpose

    Expresses Non-targeting sgRNA3 control in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    Non-targeting sgRNA3 (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP - FLAG-APEX2- hs Lamin B1

    Plasmid

    #139442

    Purpose

    APEX2-Lamin B1 expression plasmid

    Depositor

    Yixian Zheng

    Article

    Tran et al bioRxiv 2020.02.05.935635

    Insert

    LMNB1 (LMNB1 Human)
    

    Use

    Tags

    3xGGGGS linker (between APEX2 and Lamin B1), APEX…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBABEpuro-ARAF

    Plasmid

    #51076

    Purpose

    mammalian expression of wt ARAF

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. 3xFLAG-dCas9/pMXs-puro

    Plasmid

    #51240

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKG-GW1

    Plasmid

    #203163

    Purpose

    Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. As0

    Plasmid

    #123158

    Purpose

    Arsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR-ABS67, PhrpLE, PrinA

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAVS1-Puro-CAG-GCaMP6s

    Plasmid

    #120896

    Purpose

    Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

    Depositor

    Xiaojun Lian

    Article

    Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

    Insert

    GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMXs-hFOXH1

    Plasmid

    #59183

    Purpose

    Forced expression of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    FOXH1 (FOXH1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCold TDP43 Q331K

    Plasmid

    #27454

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa and His6

    Expression

    Bacterial

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Promoter

    Available since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

35. CDK1as_T2A_puromycin

    Plasmid

    #118595

    Purpose

    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

    Depositor

    William Earnshaw

    Article

    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

    Insert

    CDK1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-FLAG-MTOR-V2006I

    Plasmid

    #69012

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR (MTOR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    V2006I

    Promoter

    CMV

    Available since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEX-GFP-hLC3WT

    Plasmid

    #24987

    Depositor

    Isei Tanida

    Article

    Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

    Insert

    a wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDRFLIP38-AtLEA4-5

    Plasmid

    #178189

    Purpose

    SED1 biosensor for expression in Saccharomyces cerevisiae

    Depositor

    Cesar Cuevas-Velazquez, Jose Dinneny

    Article

    Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

    Insert

    SED1 biosensor
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pYDE009

    Plasmid

    #194151

    Purpose

    Delivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    tetR-tetP-dcas9-rrnB T1-T7Te
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetp

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR-p38KTRmCerulean3

    Plasmid

    #59149

    Purpose

    ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge