We narrowed to 8,512 results for: sgrna

1. PX458_CEBPB_1

   Plasmid

   #64036

   Purpose

   Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PX458_CEBPB_2

   Plasmid

   #64047

   Purpose

   Expresses gRNA against human CEBPB along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA-GFP_rescue_construct

   Plasmid

   #190641

   Purpose

   Puromycin-selectable expression of GFP in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   EGFP
   

   Use

   Tags

   3xHA

   Expression

   Insect

   Mutation

   Promoter

   Actin-5c

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHX_gRNA_hPAX7_6-9S

   Plasmid

   #160453

   Purpose

   sgRNA targeting human PAX7 3' UTR with 17bp spacer

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7_6-9S
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. lentiCRISPRv2-Blast-mU6

   Plasmid

   #206806

   Purpose

   pLentiCRISPRv2 variant with mU6 promoter driving the sgRNA, and a Blasticidin resistance cassette

   Depositor

   Jolanda Van Leeuwen

   Article

   Unlu et al Genome Med. 2023 Oct 12;15(1):78. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCY_gRNA_hPAX7-Pro_C6

   Plasmid

   #160457

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C6
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCY_gRNA_hPAX7-Pro_C5

   Plasmid

   #160456

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C5
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCY_gRNA_hPAX7-Pro_C4

   Plasmid

   #160455

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCY_gRNA_hPAX7-Pro_C3

    Plasmid

    #160454

    Purpose

    sgRNA targeting human PAX7 promoter

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7-Promoter_C3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSB700 lenti gRNA EBFP2

    Plasmid

    #167905

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LRT2B-AdGO

    Plasmid

    #136910

    Purpose

    Lentivirus expressing AdGO sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgAdGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTJV1Sc2-tetA

    Plasmid

    #166982

    Purpose

    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    CspRecT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pVanCC

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMH183

    Plasmid

    #122856

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiCRISPRv2-Stuffer-HepmTurquoise2

    Plasmid

    #192826

    Purpose

    Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

    Depositor

    Kristin Knouse

    Article

    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

    Insert

    mTurquoise2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

    Available since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCBLgRNA

    Plasmid

    #199726

    Purpose

    Gateway entry vector for single gRNA under OsU3 promoter.

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    sgRNA scaffold
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458_CREB1_2

    Plasmid

    #64940

    Purpose

    Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMM731

    Plasmid

    #68896

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX458_GABPA_2

    Plasmid

    #64255

    Purpose

    Expresses gRNA against human GABPA along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lenticrispr-wt-ltr-puro

    Plasmid

    #173428

    Purpose

    Expresses Cas9 and guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. lentiguide-puro-wt-ltr

    Plasmid

    #182635

    Purpose

    Expresses guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PX458_GABPA_1

    Plasmid

    #64254

    Purpose

    Expresses gRNA against human GABPA along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUCSh3-mRFP

    Plasmid

    #170983

    Purpose

    Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    mRFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMM705

    Plasmid

    #68892

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. px459 EQR

    Plasmid

    #101732

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    VQR

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-U6-CB2gRNA-CBh-mCherry

    Plasmid

    #91948

    Purpose

    Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PX458_ATF1_1

    Plasmid

    #64690

    Purpose

    Expresses gRNA against human ATF1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICSL1P3_AtU626Ter26

    Plasmid

    #191771

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMM763

    Plasmid

    #68900

    Purpose

    IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    G1104E

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pU6Rosa-CAG-Cas9

    Plasmid

    #118998

    Purpose

    Mammalian expression of Rosa26 sgRNA, Cas9 and BFP

    Depositor

    Ralf Kuehn

    Article

    Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHpgRNA50

    Plasmid

    #160128

    Purpose

    To express a sgRNA with constant targeting LSC2 by RNA pol II promoter PTEF1

    Depositor

    Yongjin Zhou

    Article

    Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

    Insert

    PTEF1-HH-gRNA-LSC2- HDV-TAMO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LRT2B-GO2

    Plasmid

    #136909

    Purpose

    Lentivirus expressing GO2 sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgGO2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

    Plasmid

    #124287

    Purpose

    sgRNA against human Col7A1

    Depositor

    Csaba Vizler, Katalin Josvay

    Insert

    Collagen type VII alpha 1 chain (COL7A1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

34. LRT2B-GO

    Plasmid

    #136908

    Purpose

    Lentivirus expressing GO sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgGO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    hU6

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pEPOR2KN0040

    Plasmid

    #117607

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEPOR2KN0038

    Plasmid

    #117605

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBC2102-reci

    Plasmid

    #117171

    Purpose

    U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    reci_wtCas9 without NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6 Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMH184

    Plasmid

    #122857

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEPOR2KN0039

    Plasmid

    #117606

    Purpose

    Level2 MoClo construct, containing level1 plant expression cassettes for AsCas12a(pEPOR1CB0014) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0028)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    [35S:AsCas12a(pEPOR1CB0014) ] +[AtU6-26:sgRNA]
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMM725

    Plasmid

    #68895

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL3), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge