We narrowed to 43,236 results for: cha

1. pHRdSV40_scFv_GCN4_sfGFP_p65-hsf1_GB1_NLS

   Plasmid

   #79372

   Purpose

   Recruits p65-hsf1 to a compatible Cas9 protein for transcriptional activation

   Depositor

   George Church

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   GCN4-sfGFP-p65-hsf1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT85_SpdCas9_pmcDA1

   Plasmid

   #204614

   Purpose

   CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. KG#121

   Plasmid

   #110879

   Purpose

   Expresses the C. elegans unc-31 cDNA pan-neuronally

   Depositor

   Kenneth Miller

   Article

   Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

   Inserts

   rab-3 promoter
   unc-31 cDNA
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Missing first 408bp

   Promoter

   Available since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB22

   Plasmid

   #122244

   Purpose

   Lentivirus delivery for stable expression of AsCas12a-VPR

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Insert

   mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
   

   Use

   Lentiviral

   Tags

   NLS and VPR activator

   Expression

   Mutation

   Promoter

   EFs

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRMTK-clo6

   Plasmid

   #203957

   Purpose

   Cloning plasmid containing the kanamycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Kanamycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAct:dCas9-VP64

   Plasmid

   #78901

   Purpose

   Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNC1011

   Plasmid

   #42898

   Depositor

   Beverly Errede

   Article

   Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

   Insert

   3 tandem copies of GFP*
   

   Use

   Yeast integrating

   Tags

   Expression

   Mutation

   changed Phenylalanine 64 to Leucine, Serine 65 t…

   Promoter

   None

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRMTK-clo10

   Plasmid

   #203961

   Purpose

   Cloning plasmid containing the erythromycin resistance gene insert

   Depositor

   Nathan Crook

   Article

   van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

   Insert

   Erythromycin Resistance Gene
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   N/A

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. U6-spTRE3G-CMV-mTagBFP2

   Plasmid

   #102854

   Purpose

   A plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFP

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-TRE3G-sgRNA; mTagBFP2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_TRE_AP4-PDGFR_EF1a_eBFP2

    Plasmid

    #186306

    Purpose

    Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    AP4-PDGFR
    eBFP2
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-sCS4-2T

    Plasmid

    #28250

    Depositor

    Yawen Bai

    Article

    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

    Insert

    single chain Cse4-Scm3-H4 with 2 Thrombin site
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A thrombin cutting site between Cse4 and Scm3. An…

    Promoter

    Available since

    July 12, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pCfB8792

    Plasmid

    #126910

    Purpose

    Template for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 gene

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pES.HSV2.3a

    Plasmid

    #69593

    Purpose

    TCR a chain construct for generation of gBT-I mice

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR alpha chain cDNA from clone HSV2.3 (Tcra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H-2Kb

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCfB5191

    Plasmid

    #126911

    Purpose

    Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    X-4 and XII-5 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET/LEGH

    Plasmid

    #108226

    Purpose

    Bacterial expression of EGFP with N-terminal lanthanide binding tag and C-terminal His6 tag

    Depositor

    Didier Picard

    Article

    Bhattacharya et al Sci Rep. 2018 Feb 12;8(1):2801. doi: 10.

    Insert

    LBT-EGFP-His6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, lacO

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNM3749

    Plasmid

    #154108

    Purpose

    loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Inserts

    LoxP-SEC-LoxP
    FRT::rpl-28p::GFP::his-58::FRT3
    

    Use

    Cre/Lox; Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKH-MoHyPer

    Plasmid

    #78267

    Purpose

    Express HyPer-AS sensor that change excitation wavelength with the presence of hydrogen peroxide.

    Depositor

    Nicole Donofrio, Jeffrey Caplan

    Article

    Huang et al Mol Plant Pathol. 2016 Mar 7. doi: 10.11

    Insert

    MoHyper
    

    Use

    Express in magnaporthe

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Rp27

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pCASP-HilA empty vector

    Plasmid

    #153333

    Purpose

    Triggers HilA overexpression from Salmonella upon induction with arabinose without any synthetic protein expressed

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Type

    Empty backbone

    Use

    Salmonella expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. bactin:GFP-DCX

    Plasmid

    #105961

    Purpose

    transgenesis, ubiquitous expression

    Depositor

    Caren Norden

    Article

    Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

    Insert

    DCX (DCX Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pASH3 [pmyo-3::BeCNG1-YFP]

    Plasmid

    #168167

    Purpose

    Expression of BeCNG1-YFP in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

    Insert

    BeCNG1-YFP
    

    Use

    Tags

    YFP

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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