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pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep

Plasmid

#213542

Purpose

AAV viral vector packaging plasmid that expresses CoV2

Depositor

Brandon Harvey, Christopher Richie

Insert

CoV2 Orf3a-2xStrep (ORF3a Severe acute respiratory syndrome coronavirus 2, Human)

Use

AAV

Promoter

EF1a

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep

Plasmid

#213543

Purpose

AAV viral vector packaging plasmid that expresses CoV2

Depositor

Brandon Harvey, Christopher Richie

Insert

CoV2 protE-2xStrep (E Severe acute respiratory syndrome coronavirus 2, Human)

Use

AAV

Promoter

EF1a

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJH2157

Plasmid

#179204

Purpose

Pacr-2::Ca -insensitive D3cpv unc-54 3' UTR C.elegans neural expression of Pacr-2 calcium D3cpv

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

D3cpv

Expression

Worm

Promoter

Pacr-2

Available Since

Feb. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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AP-VAMP1(X)

Plasmid

#167999

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(L56-L72)-T7PAP

Use

Synthetic Biology

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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AP-VAMP5

Plasmid

#168001

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP5(L28-L44)-T7PAP

Use

Synthetic Biology

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-2

Plasmid

#164859

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #2 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-2

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-3

Plasmid

#164860

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #3 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-3

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 R59K

Plasmid

#112652

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 R59K

Expression

Yeast

Mutation

R59K (GAP-dead)

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Gcs1 R54K

Plasmid

#112647

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Gcs1 R54K

Expression

Yeast

Mutation

R54K

Promoter

Met25

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a Q146F

Plasmid

#75259

Purpose

Q146F mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a

Tags

His

Expression

Yeast

Mutation

Q146F

Promoter

Met25

Available Since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a T158K

Plasmid

#75262

Purpose

T158K mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a T158K

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a K153Q

Plasmid

#75261

Purpose

K153Q mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a K153Q

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 Ret2LD2a N152T

Plasmid

#75260

Purpose

N152T mutation

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2a N152T

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GCN4cc-Erp2CT

Plasmid

#73923

Purpose

GST fusion- coiled coil- Erp2 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp2 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1- Mst7-Erp2CT

Plasmid

#73922

Purpose

GST fusion- Erp2 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp2 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GCN4cc-Erp1CT

Plasmid

#73921

Purpose

GST fusion- coiled coil- Erp1 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp1 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1- Mst7-Erp1CT

Plasmid

#73920

Purpose

GST fusion- Erp1 cytoplasmic tail

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp1 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 δCLD

Plasmid

#73876

Purpose

δCLD - His tagged (2α helices+μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

δCLD

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD

Plasmid

#73875

Purpose

Ret2LD - His tagged (2α helices+μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD2α

Plasmid

#73873

Purpose

Ret2LD2α - His tagged (μHD deleted)

Depositor

Blanche Schwappach

Article

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD2α

Tags

His

Expression

Yeast

Promoter

Met25

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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