We narrowed to 11,754 results for: pcDNA

1. pcDNA3 Connexin43-APEX

   Plasmid

   #44439

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

   Insert

   Connexin43 fused to pea APEX
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   K14D, W41F, E112K mutations on APEX

   Promoter

   CMV

   Available since

   May 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-DTXK-lumitoxin

   Plasmid

   #51691

   Purpose

   Expression vector for DTXK-lumitoxin

   Depositor

   Edward Boyden

   Article

   Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

   Insert

   DTXK-lumitoxin
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY013 (pcDNA3.1-hEeCpf1)

   Plasmid

   #69985

   Purpose

   Expresses humanized EeCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hEeCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3-PKCZeta KR HA Tagged

   Plasmid

   #24608

   Depositor

   Jeff Wrana

   Article

   Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

   Insert

   PKCZeta (Prkcz Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K281R mutation in the ATP binding domain causing …

   Promoter

   Available since

   June 7, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pcDNA3-Lyn-GR-AKAR3

   Plasmid

   #173016

   Purpose

   Plasma membrane-targeted variant of improved green-red FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Mo et al Nat Commun. 2020 Apr 15;11(1):1848. doi:

   Insert

   Lyn-GR-AKAR3
   

   Use

   Tags

   EGFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5-FRT-TO-Nsp5

   Plasmid

   #157685

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp5 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pCDNA3 Flag MKK7b2

   Plasmid

   #14623

   Depositor

   Roger Davis

   Article

   Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

   Insert

   MKK7b2 (Map2k7 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3-PDE4D2cat-ICUE4

   Plasmid

   #181847

   Purpose

   ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
   

   Use

   Tags

   ECFP, PDE4D2(86-418), and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HSF2BP_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176947

   Purpose

   Mammalian expression vector encoding HSF2BP and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   HSF2BP (HSF2BP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-P2X2-CFP

    Plasmid

    #22401

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

11. ciCas9(L22)_pcDNA5

    Plasmid

    #100551

    Purpose

    Expresses ciCas9(L22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

    Insert

    ciCas9(L22)
    

    Use

    CRISPR; Frt, flp-in

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-nAChr-Alpha6

    Plasmid

    #48812

    Purpose

    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

    Depositor

    Henry Lester

    Article

    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

    Insert

    nAChr-alpha6 (Chrna6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5FRT/TO-Strep/HA-UBE2QL1

    Plasmid

    #124665

    Purpose

    Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Use

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-Cx43-Myc

    Plasmid

    #60693

    Purpose

    mammalian expression of Myc-tagged rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. Kohinoor-Paxillin/pcDNA3

    Plasmid

    #67775

    Purpose

    Paxilin-targetted Kohinoor for mammalian cell expression

    Depositor

    Takeharu Nagai

    Article

    Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

    Insert

    Kohinoor
    

    Use

    Tags

    Paxillin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-Beta2-PAmcherry1

    Plasmid

    #114190

    Purpose

    studying clustering of Beta2 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Adrenergic receptor beta 2 (ADRB2 Human)
    

    Use

    Tags

    3HA, FLAG, and PAmcherry1

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO DNAJA1

    Plasmid

    #19464

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJA1 (DNAJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D244G

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT-TO-FH-Nsp1

    Plasmid

    #157680

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCDNA3.1-hMcl-1(T163A)

    Plasmid

    #25376

    Depositor

    Roger Davis

    Article

    Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

    Insert

    hMCL1 (MCL1 Human)
    

    Use

    Tags

    V5 and His tag

    Expression

    Mammalian

    Mutation

    changed threonine 163 to alanine

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pY019 (pcDNA3.1-hPmCpf1)

    Plasmid

    #69991

    Purpose

    Expresses humanized PmCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPmCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only