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pET26b(+)-ATI_QconCAT_1
Plasmid#163957PurposeExression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.DepositorInsertATI QconCAT
UseTagspolyhistidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xnls-cpmTq2-Calcium-lifetime-sensor
Plasmid#129626PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-cpmTq2-Calcium-lifetime-sensor
UseTags3xNLSExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
tetO-FHL2
Plasmid#170686Purposedoxycycline-inducible overexpression of FHL2DepositorInsertFHL2 (FHL2 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xHA-MKK4-EE
Plasmid#47577PurposeBacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4DepositorInsertMKK4-EE (MAP2K4 Human)
UseTags3xHA and GSTExpressionBacterialMutationLacks the first 36 residues of the RefSeq protein…PromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET30a-His6-ABP-LOXCATmut
Plasmid#139972PurposeA control for His6-ABP-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)DepositorInsertA fusion of lactate oxidase from A. viridans and catalase from E. coli
UseTagsHis6ExpressionBacterialMutationMutation in H265A and R268A in LOX (used original…PromoterT7Available sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YD EGFP
Plasmid#187264PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsEGFPExpressionMutation3YD (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
tetO-ETS2
Plasmid#170685Purposedoxycycline-inducible overexpression of ETS2DepositorInsertETS2 (ETS2 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
Plasmid#165493PurposeNeuronal expression of PACmn with dark Venus, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activityDepositorInsert2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
UseAAVTagsdarkVenusExpressionMammalianMutationPromoterCaMKIIAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 mCherry-hCortKD-Flcortmut3YD
Plasmid#187266PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryExpressionMutation3YD (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YF mCherry
Plasmid#187265PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryExpressionMutation3YF (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-383)-GFP-SSPB(milli)
Plasmid#174626PurposeConstitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)DepositorInsertKIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)
UseTagsGFP-SSPB(milli)ExpressionMammalianMutationmmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…PromoterChicken beta-actinAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Granulin 2+linker3
Plasmid#176920Purposeexpress Granulin 2 with linker 3 in mammalian cellsDepositorInsertGranulin 2+linker3 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
tetO-ESRRG
Plasmid#170684Purposedoxycycline-inducible overexpression of ESRRGDepositorInsertESRRG (ESRRG Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin GC
Plasmid#68818PurposeFlag tagged mammalian expression of GC domainDepositorInsertGephyrin (Gphn Rat)
UseTags3xFLAGExpressionMammalianMutationNILPromoterCMVAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBItetDT240GFP
Plasmid#96904Purposetet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 locatedDepositorInsertEGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
UseTetracycline responsive and bidirectionalTagsExpressionMammalianMutationPromotertetracycline responsive and bidirectionalAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54(R159E)
Plasmid#172453PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag R159E mutantDepositorInsertTPD54 (TPD52L2 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationchanged Arginine 159 to Glutamic AcidPromoterCMVAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Paragranulin-no linker
Plasmid#176917Purposeexpress Paragranulin without linker 1 in mammalian cellsDepositorInsertParagranulin (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_Paragranulin+linker 1
Plasmid#176915Purposeexpress Paragranulin with linker 1 in mammalian cellsDepositorInsertParagranulin + linker 1 (GRN Human)
UseTagsTwin-Strep and FLAG tags encoded after Signal Pep…ExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CCre-MBP4-WPRE
Plasmid#193919PurposeExpresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)DepositorInsertCCre-MBP4
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only
