We narrowed to 231,372 results for: Des

1. pEJS436-pCSDest2-AcrIIC2Nme

   Plasmid

   #85712

   Purpose

   Mammalian expression of Type II-C anti-CRISPR protein AcrIIC2Nme from Neisseria meningitidis

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Insert

   Type II-C anti-CRISPR AcrIIC2Nme
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.2/v5-DEST hGlut9

   Plasmid

   #18088

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   Glut9 (SLC2A9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pDEST27-GST-hADAM10

   Plasmid

   #189553

   Purpose

   Protein expression ADAM10 in mammalian cells.

   Depositor

   Haian Fu

   Insert

   ADAM metallopeptidase domain 10 (ADAM10 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST-DHFR F[3]-C (LEU2)

   Plasmid

   #177796

   Purpose

   Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Use

   Tags

   DHFR F[3]

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST47-ARF1-GFP

   Plasmid

   #67390

   Purpose

   Mammalian expression of hArf1 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF1 (ARF1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pDEST47-ARL11-GFP

   Plasmid

   #67407

   Purpose

   Mammalian expression of hArl11 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL11 (ARL11 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   bp 442 T to C; aa C148R

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pDEST40-ARF4-V5-His6

   Plasmid

   #67434

   Purpose

   Mammalian expression of hArf4 with C-term V5 and His6 tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARF4 (ARF4 Human)
   

   Use

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCheck2 Dest (R1-R2) #BGV129

   Plasmid

   #48955

   Purpose

   Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Luciferase; Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   SV40

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pDEST47-ARL4-GFP

   Plasmid

   #67398

   Purpose

   Mammalian expression of hArl4 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL4 (ARL4A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   bp 105 A to T; silent mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pDEST40-ARF1-V5-His6

    Plasmid

    #67432

    Purpose

    Mammalian expression of hArf1 with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pDEST26 Ago2 S387A

    Plasmid

    #59905

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    S387A, phospho-null alanine

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST40-2XHA-Src-S4-UD

    Plasmid

    #140304

    Purpose

    Expresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH4 and UD regions

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST40-2XHA-Src-S4-KD

    Plasmid

    #140296

    Purpose

    Expresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST40-2XHA-Src-S4-UD-KD

    Plasmid

    #140300

    Purpose

    Expresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X HA

    Expression

    Mammalian

    Mutation

    lacks the SH3 and SH2 domains

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST26-his-hSFRP1

    Plasmid

    #189539

    Purpose

    Protein expression of SFRP1 in mammalian cells.

    Depositor

    Haian Fu

    Insert

    secreted frizzled related protein 1 (SFRP1 Human)
    

    Use

    Tags

    his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 366 pTol2-CherryDest

    Plasmid

    #73487

    Purpose

    promoterless Tol2 vector with mCherry tag upstream of and in frame with attR1/attR2 cassette and late SV40 polyadenylation signal

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    mCherry-attR1/attR2
    

    Use

    Tags

    Expression

    Mutation

    H30R (please see depositor comments below)

    Promoter

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT-Rex Dest30 dn-cHSF1

    Plasmid

    #134736

    Purpose

    Mammalian expression vector for inducible expression of a constitutively active dominant-negative HSF1

    Depositor

    Matthew D. Shoulders

    Article

    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

    Insert

    dn-cHSF1 (HSF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 186-202, 379−529

    Promoter

    CMV/TO

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-EB-Hyg-R4R3

    Plasmid

    #139520

    Purpose

    Provides episomal replication of the destination vector in primate cells

    Depositor

    Minoru Ko

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDest472 SHP-1 S591D

    Plasmid

    #21806

    Depositor

    Stephen Shaw

    Article

    Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

    Insert

    SHP-1 (PTPN6 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Serine 591 to Aspartic Acid

    Promoter

    Available since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pDEST47-ARF4-GFP

    Plasmid

    #67392

    Purpose

    Mammalian expression of hArf4 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V53A, L123P and M134I mutations were unintended P…

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only