We narrowed to 32,111 results for: Eng

1. pIALD2HMGr

   Plasmid

   #185867

   Purpose

   Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETDuet1-LanCL2-H264A-C-Ter-His

   Plasmid

   #154187

   Purpose

   To express LanCL2-H264A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His264->Ala264

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-15b-LanCL1-H219A

   Plasmid

   #154190

   Purpose

   To express LanCL1-H219A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His219->Ala219

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-15b-LanCL1-C322A

   Plasmid

   #154191

   Purpose

   To express LanCL1-C322A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Cys322->Ala322

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMAL-p2x-LanCL2

   Plasmid

   #154192

   Purpose

   To express MBP-LanCL2 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Maltose binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV-TTBG

   Plasmid

   #115232

   Purpose

   Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLV-TTBL

   Plasmid

   #115233

   Purpose

   Lentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   mTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PLKO.1-Cd155 shRNA2

   Plasmid

   #166488

   Purpose

   Mouse Cd155 RNAi

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   shRNA for mouse Cd155 (Pvr Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PLKO.1-Cd155 shRNA1

   Plasmid

   #166487

   Purpose

   Mouse Cd155 RNAi

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   shRNA for mouse Cd155 (Pvr Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCherry-N1-MOB1

    Plasmid

    #166477

    Purpose

    Expresses fusion of mCherry and MOB1

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mCherry, MOB1 (MOB1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PatoM-OG241

    Plasmid

    #72842

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Shine-Dalgarno sequence provided in OG241. OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoM
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PatoM

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEXP5-NT-7th R

    Plasmid

    #42573

    Depositor

    Ivan Korendovych

    Article

    Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

    Insert

    mutated calmodulin gene
    

    Use

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Isole…

    Promoter

    T7

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV_Cas9–superNLS

    Plasmid

    #232430

    Purpose

    Expression plasmid for the SpCas9 nuclease with superNLS

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    Cas9–superNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV_iPE-C_P2A_Csy4

    Plasmid

    #232428

    Purpose

    Expression plasmid for iPE-C prime editor RNPs with additional Csy4 protection

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    iPE-C–P2A–Csy4
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CRISPR-SB

    Plasmid

    #177936

    Purpose

    Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-SpRYc-ABE8e-EGFP

    Plasmid

    #208340

    Purpose

    Expresses SpRYc-ABE8e in mammalian cells

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    ecTadA(8e)-nSpRYc
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CMV

    Available since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-FGFR3__TACC3

    Plasmid

    #116818

    Purpose

    Lentiviral expression of FGFR3__TACC3 fusion

    Depositor

    Kenneth Scott, Gordon Mills

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    FGFR3__TACC3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV_CreNLS_PP7

    Plasmid

    #232439

    Purpose

    expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    Cre–MYC-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUNIV-SIG-5HTR4D100A

    Plasmid

    #16312

    Depositor

    Bruce Conklin

    Article

    Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

    Insert

    h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Added FLAG tag; Aspartic Acid 100 changed to Alan…

    Promoter

    Available since

    Jan. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pDisplay-GRAB_NPY1.0

    Plasmid

    #208675

    Purpose

    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge