We narrowed to 8,728 results for: Set

1. pcDNA3.2-DEST-MatryoshCaMP6s

   Plasmid

   #100025

   Purpose

   Mammalian expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   MatryoshCaMP6s
   

   Tags

   V5 Epitope Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TAC-OK+9MS-Cas9

   Plasmid

   #120354

   Purpose

   piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OK+9MS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [1-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB2-neo-TET

   Plasmid

   #51287

   Purpose

   expresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

   Depositor

   Astrid Roy-Engel

   Article

   Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

   Insert

   sine B2
   

   Tags

   neoTET cassette with a tetrahymena self-splicing …

   Expression

   Mammalian

   Promoter

   enhancer from 7SL gene

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PpyCAG-NANOG-IB

   Plasmid

   #60440

   Purpose

   Transient human NANOG expression

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   NANOG (NANOG Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD_B2101

   Plasmid

   #91060

   Purpose

   Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
   

   Use

   CRISPR

   Promoter

   35S

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR P2R-P3-SV40 polyA signal

   Plasmid

   #48627

   Purpose

   Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.

   Depositor

   Tomas Santalucia, Anna Planas

   Article

   Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:

   Insert

   SV40 early polyadenylation signal cassette
   

   Use

   Gateway entry vector

   Promoter

   none

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKan-PRFA1-9myc-AID*(N)

   Plasmid

   #99528

   Purpose

   N-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-117) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Promoter

   RFA1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pScalps_puro_mIL-2Rα

   Plasmid

   #59917

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pKan-PCUP1-9myc-AID*(N)

   Plasmid

   #99527

   Purpose

   N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-117) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Promoter

   CUP1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. BtuF

    Plasmid

    #92209

    Purpose

    Expression of BtuF which is the vitamin B12 binding protein for BtuCD ABC Transporter

    Depositor

    Douglas Rees

    Article

    Borths et al Proc Natl Acad Sci U S A. 2002 Dec 24;99

    Insert

    BtuF (btuF )
    

    Tags

    6x His Tag and PelB Signal Sequence

    Expression

    Bacterial

    Mutation

    BtuF signal sequence removed and uses the PelB si…

    Promoter

    T7 Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATP1A1_G3_Dual_sgRNA

    Plasmid

    #173202

    Purpose

    Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + user-specified sgRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMOD_B2112

    Plasmid

    #91064

    Purpose

    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    PvUbi1

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMOD_B2203

    Plasmid

    #91066

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    CmYLCV

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. ATP1A1_G2_Dual_sgRNA

    Plasmid

    #173201

    Purpose

    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. JN302: pMVP/SB/Puro-DEST

    Plasmid

    #121865

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

    Plasmid

    #211904

    Purpose

    Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

    Depositor

    Yannick Doyon

    Article

    Levesque et al bioRxiv 2023.09.14.557485

    Insert

    CD19-CAR-28z-2A-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hPGK1

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAsCpf1(TATV)(BB) (pY221)

    Plasmid

    #89354

    Purpose

    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1 RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CBh

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOD_B2103b

    Plasmid

    #91062

    Purpose

    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Promoter

    CmYLCV

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. scAAV-Mlc2v-MPRA

    Plasmid

    #182649

    Purpose

    Self complementary AAV vector for high-throughput in vivo measurement of enhancer activity by massively parallel reporter assay

    Depositor

    William Pu

    Article

    Akerberg et al Nat Commun. 2019 Oct 28;10(1):4907. doi:

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    rat Mlc2v

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pS44i8GM

    Plasmid

    #207527

    Purpose

    Conditionally-replicating in Pseudomonas vector, medium-copy-number; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP; SmR/SpR

    Depositor

    Pablo Ivan Nikel

    Article

    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

    Insert

    oriV(pRO1600/ColE1), xylS, Pm→repA, P14b with a translational BCD2 coupler from BG35 (53)→msfGFP
    

    Use

    CRISPR; Pseudomonas vector

    Available Since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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