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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,445 results for: grn

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Showing: 3001 - 3020 of 15445 results

  12. lentiCRISPR - Amplicon, JAK2 sgRNA 2

    Plasmid
    #70660
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Publication
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Oct. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. LX-miR

    Pooled library
    #112200
    Purpose
    Targets 1,594 primary human miRNA genes. The library contains 4-5 gRNAs per gene and 1,000 non-targeting control gRNAs for a total of 8,382 gRNAs.
    Depositor
    Ren-Jang Lin
    Publication
    Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do
    Expression
    Mammalian
    Species
    Homo sapiens
    Use
    Lentiviral
    Available since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  16. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    139087-plasmid-map-sequence-id-273883

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Showing: 3001 - 3020 of 15445 results