We narrowed to 44,063 results for: tro
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Plasmid#227380PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only
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FUS-3YS-miRFP670-TRF1
Plasmid#227379PurposeAdhesion module, creates 'seed' at telomereDepositorAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-marmoset cGASdeltaN-HA
Plasmid#186863PurposeDoxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transductionDepositorInsertMarmoset cGAS truncation mutant (160-547 a.a.) with C-terminal HA tag
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-WHG cGASdeltaN-HA
Plasmid#186868PurposeDoxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transductionDepositorInsertWhite-handed gibbon cGAS truncation mutant (193-557 a.a.) with C-terminal HA tag
UseRetroviralTagsHAMutationN-terminal truncationPromoterTRE3GAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSuper DGK alfa mouse
Plasmid#224573PurposeNegative Control for expression downregulation of DGK alfa human in human cellsDepositorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zed_vector_tol2_-2.1prox1a_2:EGFP_XCA:DsRed2
Plasmid#218208PurposeZebrafish -2.1prox1a_2 (not mouse conserved) enhancer reporter in the facial lymphatics at 5 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vectorDepositorInsert-2.1prox1a_2
UseZebrafish expressionAvailable SinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-POLL
Plasmid#215141PurposeExpresses mCherry-POLL in mammalian cells from a retroviral vector.DepositorInsertPOLL
UseRetroviralTagsmCherryExpressionMammalianPromoterCMVAvailable SinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMX-IG-ATG3 (K62D/K64D)
Plasmid#212031PurposeExpresses untagged ATG3 (K62D/K64D) in mammalian cellsDepositorAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
Qt-FLAG-TGA-rt9us-antiGFP
Plasmid#206954PurposeMammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)DepositorInsertQtEnc, antiGFP-intrabody
ExpressionMammalianAvailable SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLINE1ΔORF1-2-scFvFc
Plasmid#206022PurposeThe ORF1 and ORF2 sequences were removed from pLINE1-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
ExpressionMammalianAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLINE1mORF1-ΔORF2-scFvFc
Plasmid#206023PurposeThe base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFcDepositorInsertmouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
ExpressionMammalianAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Bn CAAX pcDNA3
Plasmid#206088PurposeCav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motifDepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Qt-FLAG-TAA-rt20s-antiGFP
Plasmid#206955PurposeMammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)DepositorInsertQtEnc, antiGFP-intrabody
ExpressionMammalianAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shFYN1/GFP4_Seq_B
Plasmid#206135PurposeKnockdown of FYNDepositorInsertFYN shRNA (Fyn Mouse)
ExpressionMammalianAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shFYN1/GFP4_Seq_A
Plasmid#206134PurposeKnockdown of FYNDepositorInsertFYN shRNA (Fyn Mouse)
ExpressionMammalianAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-IGFBP2-Clover
Plasmid#191007PurposeGateway entry vector with IGFBP2 tagged with CloverDepositorAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-POFUT2-SVA
Plasmid#202831PurposepGL3 luciferase reporter with POFUT2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertPOFUT2 intronic SVA
UseLuciferaseExpressionMammalianPromoterSV40Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-HTT-SVA
Plasmid#202833PurposepGL3 luciferase reporter with HTT SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertHTT intronic SVA
UseLuciferaseExpressionMammalianPromoterSV40Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-luciferase-reporter-CHAF1B-SVA
Plasmid#202829PurposepGL3 luciferase reporter with CHAF1B SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expressionDepositorInsertCHAF1B intronic SVA
UseLuciferaseExpressionMammalianPromoterSV40Available SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet_Seq1.2
Plasmid#201404PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/shCol1a1/Scarlet-Seq1.1
Plasmid#201401PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/Col1a1/GFP4_Seq 1.1
Plasmid#201400PurposeKnockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertCOL1A1
ExpressionMammalianAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCEP4_HLA-cmyc-GRM3 C127A T174A-VC
Plasmid#140457PurposeMammalian expression plasmid for c-myc-tagged mGluR3 C127A and T174A mutant fused to the C-terminal half of split fluorescent Venus (VC)DepositorInsertGRM3 (GRM3 Human)
TagsSignal/leader sequence from HLA class I histocomp…ExpressionMammalianMutationCysteine 127 to alanine, Threonine 174 to alanineAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-STAT2
Plasmid#183931PurposeOptogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposureDepositorAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-Rta
Plasmid#183930PurposeOptogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposureDepositorAvailable SinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-VPR
Plasmid#183928PurposeOptogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposureDepositorAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP-VP16
Plasmid#183935PurposePCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFPDepositorInserttdPCP-GFP-VP16 (UL48 Synthetic)
TagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOLY_new
Plasmid#179276PurposeExpresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)DepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-Rac-CA(guide resistant)-2a-mcherry-U6ac-Rac guides
Plasmid#168244Purpose"Rescue Rac-CA expression in neutrophil specific knockout"DepositorInsertRac(guide resistant)-CA
UseZebrafish expressionTagsmcherryPromoterLyzCAvailable SinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Halo-WIPI2B H85E K88E I92E
Plasmid#175033PurposeExpresses WIPI2B (H85E K88E I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1DepositorInsertWIPI2B (H85E K88E I92E) (WIPI2 Human)
TagsHaloTagExpressionMammalianMutationHistadine 85 to Glutamate (CAC to GAG); Lysine 88…PromoterCMVAvailable SinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
L2_2xNOP1gRNA-Cas9-CsA
Plasmid#136140PurposePlasmid with 2 different NOP1-gRNA. Targets 500 bp apart in the genome. To tranform Mp as CRISPR control. Contains Cas9 and HygRDepositorInsertp5-35S:HygR p5-MpU6:NOP1gRNA2 p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcryGFP-acta1-dmdGFP
Plasmid#172530PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.DepositorAvailable SinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
KIF13B
Plasmid#163907PurposeFull length mouse KIF13B for purification from insect cellsDepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
KIF13B 1-584
Plasmid#163908PurposeMotile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.DepositorAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgCTRL-2
Plasmid#83935PurposeLentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.DepositorInsertsgCTRL-2
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-sgCTRL-3
Plasmid#83936PurposeLentiviral vector expressing a control sgRNA. NOTE: This plasmid does NOT contain Cas9.DepositorInsertsgCTRL-3
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVE2_12_p40S_QfDBD_VP16AD_5xQUAS_DsRed
Plasmid#154226PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS (no CP control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialPromoterp40S (AN0465 A. nidulans)Available SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM2_31_p40S_QfDBD_VP16AD_cp_ura3_DsRed_0xQUAS
Plasmid#154231PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_ura3 (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialPromoterp40S (AN0465 A. nidulans)Available SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_9_p40S_QfDBD_VP16AD_cp_pcbAB_DsRed_0xQUAS
Plasmid#154227PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter CP_pcbAB (no QUAS control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialPromoterp40S (AN0465 A. nidulans)Available SinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only