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pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69)

Plasmid

#55212

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, High pI protein 69, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET FLAG TEV cloning vector with BioBrick polycistronic restriction sites (9L)

Plasmid

#48288

Depositor

Scott Gradia

Type

Empty backbone

Tags

FLAG

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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scFLARE2

Plasmid

#158700

Purpose

Neuronal expression of scFLARE2 component

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE2

Use

AAV

Tags

HA, Flag, VP16

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

Syn

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PCR4-Shh::lacZ-H11

Plasmid

#139098

Purpose

enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

Depositor

Len Pennacchio

Publication

Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

Shh

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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EGFP-BAF

Plasmid

#101772

Purpose

Expresses EGFP tagged BAF in human cells

Depositor

Daniel Gerlich

Publication

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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EGFP-BAF

Plasmid

#101772

Purpose

Expresses EGFP tagged BAF in human cells

Depositor

Daniel Gerlich

Publication

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Ca-FLARE (TF)

Plasmid

#92213

Purpose

Transcription factor component of FLARE

Depositor

Alice Ting

Publication

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Ca-FLARE (TF)

Plasmid

#92213

Purpose

Transcription factor component of FLARE

Depositor

Alice Ting

Publication

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

NRX-TM-Nav1.6 -CaMbp(M2)-eLOV-TEVcs(ENLYFQ▲M)-tTA-VP16

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pMJ117

Plasmid

#85997

Purpose

One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr3
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified human U6

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pDONR223-K-RAS V12

Plasmid

#31200

Purpose

Gateway donor vector containing KRAS G12V.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

K-Ras (KRAS Human)

Use

Gateway Cloning

Mutation

G12V constitutively active

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDONR223-K-RAS V12

Plasmid

#31200

Purpose

Gateway donor vector containing KRAS G12V.

Depositor

David Root

Publication

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

K-Ras (KRAS Human)

Use

Gateway Cloning

Mutation

G12V constitutively active

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pcdna3 flag - ULK1 wt

Plasmid

#27636

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse ULK1 (Ulk1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M from ULK1 removed.

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcdna3 flag - ULK1 wt

Plasmid

#27636

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse ULK1 (Ulk1 Mouse)

Tags

flag

Expression

Mammalian

Mutation

Initial M from ULK1 removed.

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Flag-HA-STAMBP

Plasmid

#22560

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

STAMBP (STAMBP Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-HA-STAMBP

Plasmid

#22560

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

STAMBP (STAMBP Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCM1.36

Plasmid

#17249

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

tbb-2 3'UTR in pDONRP2RP3

Use

Gateway Cloning

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCM1.36

Plasmid

#17249

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

tbb-2 3'UTR in pDONRP2RP3

Use

Gateway Cloning

Available since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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