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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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CA-NFAT2

Plasmid

#11102

Depositor

Anjana Rao

Article

Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

Insert

NFAT2 (Nfatc1 Mouse)

Use

Retroviral

Tags

GFP and HA

Expression

Mammalian

Mutation

Constitutively active: multiple serine to alanine…

Available Since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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pBS-HA::UPRT

Plasmid

#47110

Purpose

for subcloning UPRT into expression vectors

Depositor

Mike Cleary, Chris Doe

Article

Miller et al Nat Methods. 2009 Jun;6(6):439-41. doi:

Insert

uracil phosphoribosyltransferase

Tags

HA

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pETMCSI-T4P_Y320A

Plasmid

#122522

Purpose

Expression in BL21(DE3) PlysS of mutant T4 DNA polymerase Y320A for easy cloning and mutagenesis

Depositor

Gottfried Otting

Article

Qi et al PLoS One. 2019 Jan 23;14(1):e0211065. do

Insert

gp43 - DNA polymerase (43 Escherichia virus T4)

Expression

Bacterial

Mutation

tyrosine 320 changed to alanine

Promoter

T7 promoter

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTO128_pPZP-NATca

Plasmid

#171105

Purpose

A. tumefaciens TI plasmid encoding NATca in T-DNA

Depositor

Teresa O'Meara

Article

Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

Insert

NAT (Nourseothricin Resistance)

Use

A. tumefaciens ti plasmid

Promoter

AgTEF2

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSNAP-H3 Zeo

Plasmid

#132427

Purpose

Expressing SNAP-tagged histone H3.1 in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

Histone H3.1

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pJBL3907

Plasmid

#193140

Purpose

Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

Depositor

Julius Lucks

Article

Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

Insert

Clostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-mUbp43-His

Plasmid

#12458

Depositor

Dong-Er Zhang

Article

Malakhov et al J Biol Chem. 2002 Mar 22. 277(12):9976-8

Insert

UBP43 (Usp18 Mouse)

Tags

GST and His

Expression

Bacterial

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pDUET-ctCPR-nAMO

Plasmid

#20110

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and native Amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pJBL6392

Plasmid

#193141

Purpose

Synthetic OFF-logic Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

Depositor

Julius Lucks

Article

Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

Insert

Synthetic flipped logic Cbe riboswitch with sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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mNTERY00

Plasmid

#184110

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY00

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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mTalin2_R10

Plasmid

#111043

Purpose

Plasmid expresses the talin2 rod domain R10 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pattB-synaptobrevin-2-G4BDMD-QFAD-hsp70

Plasmid

#46110

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

G4BDMD-QFAD

Expression

Insect

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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mTalin2_R3

Plasmid

#111035

Purpose

Plasmid expresses the talin2 rod sub domain R3 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R2

Plasmid

#111034

Purpose

Plasmid expresses the talin2 rod sub domain R2 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R12

Plasmid

#111045

Purpose

Plasmid expresses the talin2 rod domain R12 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R13

Plasmid

#111046

Purpose

Plasmid expresses the talin2 rod domain R13 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R13-DD

Plasmid

#111047

Purpose

Plasmid expresses the talin2 rod domain R13 and the dimerisation domian DD.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R4

Plasmid

#111036

Purpose

Plasmid expresses the talin2 rod sub domain R4 a 4 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R11

Plasmid

#111044

Purpose

Plasmid expresses the talin2 rod domain R11 a 5 helix bundle.

Depositor

Ben Goult

Article

Gough et al FEBS Lett. 2018 May 3. doi: 10.1002/1873

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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