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Showing: 3061 - 3080 of 12674 results

  5. pRL_yEGFP_hc

    Plasmid
    #98746
    Purpose
    Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Insert
    Yeast-enhanced green fluorescent protein
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    synthetic
    Available since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98746-plasmid-map-sequence-id-189855

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  6. pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

    Plasmid
    #212705
    Purpose
    targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system
    Depositor
    Elzo de Wit
    Insert
    ZFP143-FKBPV-2HA-P2A-GFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    212705-plasmid-map-sequence-id-423954

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  9. 5' EGFP SNF-5 pXOON

    Plasmid
    #48700
    Purpose
    Encodes 5' EGFP - SNF-5 fusion construct for expression in Xenopus oocytes or Mammalian Cells
    Depositor
    Dmitri Boudko
    Insert
    snf-5 (snf-5 Nematode)
    Use
    Xenopus oocyte expression
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    48700-plasmid-map-sequence-id-70541

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  13. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    160140-plasmid-map-sequence-id-317331

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  14. p406 – TDH3-ER-FlucDM-GFP11-Ura

    Plasmid
    #177731
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Use
    Tags
    GFP11, HA, HDEL, and KAR2 Signal Sequence
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177731-plasmid-map-sequence-id-352614

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  15. p406 – TDH3-ER-FlucDM-GFP- Ura

    Plasmid
    #177732
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention and fused to eGFP at the C-terminus in yeast plasmid with URA marker
    Depositor
    Erik Böttger
    Article
    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:
    Insert
    FlucDM
    Use
    Tags
    HA, HDEL, KAR2 Signal Sequence, and eGFP
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177732-plasmid-map-sequence-id-352622

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  16. pCas9_GFP

    Plasmid
    #44719
    Purpose
    Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 24, 2013
    Availability
    Academic Institutions and Nonprofits only
    44719-plasmid-map-sequence-id-472945

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  20. TK4-NLS-EGFP

    Plasmid
    #239046
    Purpose
    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation
    Depositor
    Marius Wernig
    Inserts
    EGFP
    puroR-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2 and c-myc-NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1a
    Available since
    May 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    239046-plasmid-map-sequence-id-473412

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Showing: 3061 - 3080 of 12674 results