We narrowed to 77,030 results for: rest

1. pcDNA-Flag-LmoCas8b2

   Plasmid

   #126482

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas8b2
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-Flag-LmoCas7

   Plasmid

   #126483

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas7
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA-Flag-LmoCas5

   Plasmid

   #126484

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas5
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. bArr1-ALFA

   Plasmid

   #201496

   Purpose

   Expression of the beta-arrestin1 containing a C-terminal ALFA tag.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   bArr1-ALFA (ARRB1 Human)
   

   Tags

   ALFA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. VanGlow-GL-DSCP

   Plasmid

   #83338

   Purpose

   for Gateway cloning of enhancer elements upstream of the DSCP and GFP::Luciferase(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.

   Depositor

   Cheng-Yu Lee

   Article

   Janssens et al Dev Cell. 2017 Feb 27;40(4):367-380.e7.

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   GFP::Luciferase(nls)

   Expression

   Insect

   Promoter

   Drosophila Synthetic Core Promoter

   Available Since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-TAF15N-miRFP670-Cry2WT

   Plasmid

   #122440

   Purpose

   Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   TAF15 (TAF15 Human)
   

   Use

   Lentiviral

   Tags

   Cry2WT and miRFP670

   Expression

   Mammalian

   Mutation

   Contains only amino acids 1-208

   Promoter

   SFFV

   Available Since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

   Plasmid

   #160742

   Purpose

   Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nEPEA-(EAAAK)4-OGT(4,K852A)
   

   Tags

   HA-Tag

   Expression

   Mammalian

   Promoter

   T7/CMV

   Available Since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pODN-AAVS1-mR2-CAAX

   Plasmid

   #183871

   Purpose

   Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mRuby2-CAAX

   Expression

   Mammalian

   Mutation

   Homology arms contain point mutations to remove t…

   Promoter

   CMV

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHSG1C3

   Plasmid

   #164423

   Purpose

   single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP307-pAAV-EFS(No AgeI)-MCS3-pA

    Plasmid

    #113684

    Purpose

    EFS driven Multi Cloning Site-3 without the AgeI restriction enzyme site.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    N/A
    

    Use

    AAV

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. EC71167

    Plasmid

    #154066

    Purpose

    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    

    Use

    Synthetic Biology

    Mutation

    All BsaI, Esp3I and BPiI restriction sites were r…

    Promoter

    Zea mays Ubiquitin Promoter

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV HA hRB delta CDK + S780

    Plasmid

    #58915

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S780 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

13. OXSM_pLX307

    Plasmid

    #98357

    Purpose

    Lentiviral expression of OXSM

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    OXSM (OXSM Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe_puro_DEST_Flag_EIF5A2

    Plasmid

    #45266

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    EIF5A2 (EIF5A2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pFIN-EF1a-GFP:2A:CHER(HA)-WPRE

    Plasmid

    #44597

    Depositor

    Susan Semple-Rowland

    Article

    Verrier et al PLoS One. 2011;6(5):e20553. doi: 10.1371

    Insert

    EF1a-GFP:2A:CHER(HA)
    

    Use

    Lentiviral

    Promoter

    human elongation factor -1a (pos 2528-3971)

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSW002-PpsbA-mNeonGreen

    Plasmid

    #205017

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    mNeonGreen
    

    Expression

    Bacterial

    Mutation

    mNeonGreen is codon optimized for expression in P…

    Promoter

    PpsbA

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQStrep2-GADD45G

    Plasmid

    #31584

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    growth arrest and DNA-damage-inducible, gamma (GADD45G Human)
    

    Tags

    His and Strep

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV HA hRB delta CDK + S811

    Plasmid

    #58919

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S811 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S811 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pDONR221_SLC7A3

    Plasmid

    #132301

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

    Insert

    SLC7A3 (SLC7A3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCMV HA hRB delta CDK + S807

    Plasmid

    #58918

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with S807 restored

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only