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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,990 results for: pCAG

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Showing: 1541 - 1550 of 1990 results

  1. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

    Plasmid
    #114081
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114081-plasmid-map-sequence-id-231038

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  2. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid
    #114082
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114082-plasmid-map-sequence-id-220504

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  3. pgTS40

    Plasmid
    #169630
    Purpose
    pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    U6-driven gRNA expression and PCAG-driven SpCas9 expression
    Expression
    Mammalian
    Promoter
    U6 / PCAG
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    169630-plasmid-map-sequence-id-366781

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  4. pMP416

    Plasmid
    #134996
    Purpose
    dCas9-m6A Writer. Methylates adenines at local GATC sites. pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Depositor
    Ahmad Khalil
    Article
    Park et al Cell. 2019 Jan 10;176(1-2):227-238.e20.
    Insert
    pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A)
    Use
    Synthetic Biology
    Tags
    3xFLAG, E coli Dam(N132A), and dCas9
    Expression
    Mammalian
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    134996-plasmid-map-sequence-id-267931

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  7. pLXIN2-RFP

    Plasmid
    #99205
    Purpose
    RFP expressing bicistronic retroviral vector
    Depositor
    Saroj Mathupala
    Insert
    Red fluorescent protein
    Use
    Retroviral; Bicistronic retroviral expression vec…
    Expression
    Mammalian
    Mutation
    The dsRed cDNA was PCR amplified off AddGene plas…
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99205-plasmid-map-sequence-id-192441

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  8. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  9. pYO1100

    Plasmid
    #235707
    Purpose
    Expression of FL human ATG14L in mammalian cells
    Depositor
    Roger Williams
    Insert
    ATG14L (ATG14 Human)
    Expression
    Mammalian
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only

  10. pYO1008

    Plasmid
    #235703
    Purpose
    Expression of FL human VPS34 in mammalian cells
    Depositor
    Roger Williams
    Insert
    VPS34 (PIK3C3 Human)
    Expression
    Mammalian
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 1541 - 1550 of 1990 results