We narrowed to 5,023 results for: puc

1. TC1405

   Plasmid

   #164877

   Purpose

   BFP-preSTOP-GFP reporter

   Depositor

   Tian Chi

   Article

   Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

   Insert

   BFP-preSTOP-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-N-Zeo-V5

   Plasmid

   #207708

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. Enhanced Repressor Domain for TAL Effector (SID4X)

   Plasmid

   #43882

   Depositor

   Feng Zhang

   Article

   Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

   Insert

   SID4X Domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2013

   Availability

   Academic Institutions and Nonprofits only

4. MAPT_Halo_C_allele

   Plasmid

   #178143

   Purpose

   Donor vector for endogenous tagging of human MAPT at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (MAPT Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTn7xTS-sfGFP

   Plasmid

   #117390

   Purpose

   Tn7 tagging vector pTn7xTS with sfGFP expression scaffold

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR_full-site

   Plasmid

   #186277

   Purpose

   Reporter plasmid for full-site selection assay

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. UAS:iGluSnfr.zf1

   Plasmid

   #108356

   Purpose

   UAS driven variant of iGluSnFR for zebrafish expression

   Depositor

   Harold Burgess

   Article

   Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

   Insert

   UAS:[GlyRa1 secretion sequence]-[iGluSnFR]
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   lac

   Available since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-moxGFP-Blast

   Plasmid

   #207710

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGCG26

   Plasmid

   #82371

   Purpose

   YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PJ23119

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-3xHA-Zeo

    Plasmid

    #207740

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. qTAG-N-Zeo-3xHA

    Plasmid

    #207707

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pEF1α-Cre-2A-miRFP670nano3

    Plasmid

    #207747

    Purpose

    Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-miRFP670nano3
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pMH220

    Plasmid

    #122836

    Purpose

    dCas9 with GreenGate B-C flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    Nuclease-dead SpCas9 (dCas9), codon-optimised for Arabidopsis
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Contains D10A and H840A mutations

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. LI Intron c1

    Plasmid

    #54433

    Depositor

    Martin Parniske

    Article

    Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

    Insert

    Intron element for construction of silencing vectors (intron1 of AtWRKY33)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTX1-RNA0

    Plasmid

    #126040

    Purpose

    In-vitro-transcription of control RNA0 in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    RNA0
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-T3G-ERP2

    Plasmid

    #175188

    Purpose

    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTn7-SCOUT13.G

    Plasmid

    #201001

    Purpose

    pLv1-pUC18R6KT-miniTn7-Tc-sfGFP, TcR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    superfolder GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMLS485

    Plasmid

    #188890

    Purpose

    Worm Cas9 expression vector, Peft-3 promoter

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Peft-3::Cas9
    

    Use

    Tags

    Expression

    Worm

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TC1078-30

    Plasmid

    #164878

    Purpose

    30bp(mismatch17) cas13d gRNA for 1405 editing

    Depositor

    Tian Chi

    Article

    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

    Insert

    gRNA for TC1405
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCbeta EV (PKA catalytic subunit Cbeta)

    Plasmid

    #15311

    Depositor

    Stanley McKnight

    Article

    Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

    Insert

    PRKACB (Prkacb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only