We narrowed to 2,346 results for: puc19

1. pDCX_mEmerald_CRISPR_HDR

   Plasmid

   #239331

   Purpose

   HDR template to insert mEmerald before the stop codon in exon 7 of the human doublecortin (DCX) locus

   Depositor

   Torsten Wittmann

   Article

   Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

   Insert

   mEmerald and DCX homology arms
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMDW108

   Plasmid

   #227194

   Purpose

   Template for multi-nucleosome array

   Depositor

   Michelle Wang

   Article

   Le et al Cell. 2019 Oct 17;179(3):619-631.e15. do

   Insert

   64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. qTAG-AAVS1-PGK-Zeo

   Plasmid

   #227271

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. qTAG-C-mScarlet-EFS-Zeo

   Plasmid

   #227261

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKE011

   Plasmid

   #207912

   Purpose

   Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS018

   Plasmid

   #207911

   Purpose

   Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCS017

   Plasmid

   #207909

   Purpose

   Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS020

   Plasmid

   #207915

   Purpose

   Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKE012

   Plasmid

   #207914

   Purpose

   Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKE010

    Plasmid

    #207910

    Purpose

    Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge