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We narrowed to 30,517 results for: ide

Showing: 3101 - 3120 of 30517 results

  1. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid
    #101743
    Purpose
    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces
    Depositor
    Hermann Gaub
    Article
    Milles et al Science. 2018 Mar 30;359(6383):1527-1533
    Insert
    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    Use
    Tags
    HIS and ybbr
    Expression
    Bacterial
    Mutation
    C18S in ddFLN4, added 9F in FgßF3
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    101743-plasmid-map-sequence-id-199091

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  5. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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  6. B-evoSpCas9

    Plasmid
    #126765
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-evoSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126765-plasmid-map-sequence-id-410921

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  7. eft-3p:act-5(ptc):tbb2 3' UTR

    Plasmid
    #202331
    Purpose
    The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.
    Depositor
    Didier Stainier
    Article
    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.
    Inserts
    eft-3 promoter
    act-5(ptc)
    tbb-2 3' UTR
    Use
    Tags
    base 238 C -> A mutation causing a PTC at codo…
    Expression
    Worm
    Mutation
    Promoter
    This is a promoter sequence for C. elegans eft-3,…
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202331-plasmid-map-sequence-id-396314

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Showing: 3101 - 3120 of 30517 results