We narrowed to 16,615 results for: puromycin

1. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

   Plasmid

   #112925

   Purpose

   Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6 promoter, gRNA-SAM expression cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLKO-PTEN-shRNA-1320

   Plasmid

   #25638

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   PTEN-shRNA-1320 (PTEN Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO-PTEN-shRNA-3001

   Plasmid

   #25639

   Depositor

   Todd Waldman

   Article

   Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

   Insert

   PTEN-shRNA-3001 (PTEN Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMK382

   Plasmid

   #214373

   Purpose

   Tet-OsTIR1(F74G) (AAVS1 insertion donor)

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   Tet-OsTIR1(F74G)
   

   Expression

   Mammalian

   Promoter

   Tet ON 3G promoter

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmRFP_a_tubulin_IRES_puro2b

   Plasmid

   #21041

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Tags

   pmRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro

   Plasmid

   #136262

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   mNeonGreen-NLS

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PLKO.1-UPF1-CDS

   Plasmid

   #136037

   Purpose

   UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

   Depositor

   Anthony Leung

   Article

   Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tol2 8xSTAR-sTomato-NLS. PGK-puro

   Plasmid

   #136261

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

   Insert

   stem cell Ascl2 reporter, 8 repeats
   

   Use

   Tol2 transposase

   Tags

   sTomato-NLS

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRetrosuper USP28 shRNA-1

   Plasmid

   #15663

   Depositor

   Martin Eilers

   Article

   Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

   Insert

   USP28 shRNA (USP28 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Flag-HA-USP21

    Plasmid

    #22574

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP21 (USP21 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 783-Rx-hU6: RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228360

    Purpose

    hU6-driven expression of RfxCas13d gRNAs and arrays compatible. Contains AarI sites for guide cloning flanked by 5' DR36

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag-HA-PARP11

    Plasmid

    #22556

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    PARP11 (PARP11 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. qTAG-C-3xHA-Puro

    Plasmid

    #207729

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. qTAG-C-miniTurbo-Puro

    Plasmid

    #207725

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. MSCV JMJD3 mutant

    Plasmid

    #21214

    Depositor

    Paul Khavari

    Article

    Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

    Insert

    JMJD3 (KDM6B Human)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    H1390A

    Available Since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Phi3-GCaMP6f

    Plasmid

    #106298

    Purpose

    CMV driven GFP based calcium (Ca2+) reporter

    Depositor

    Sanford Simon

    Article

    Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

    Insert

    GCaMP6f
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMXs-IP GFP-WIPI-1

    Plasmid

    #38272

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hCdh1 (1-125)

    Plasmid

    #8508

    Depositor

    Joan Ruderman

    Article

    Crane et al J Cell Sci 2004 Dec 1;117(Pt 25):5975-83

    Insert

    hCdh1 (1-125) (FZR1 Human)
    

    Use

    Retroviral

    Tags

    His

    Expression

    Mammalian

    Mutation

    Cdh1 N-terminus. Dominant Negative mutant.

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pPB-cT3G-cERP2-HDAC2

    Plasmid

    #222553

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HDAC2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HDAC2 (HDAC2 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEF1α-Cre-PGK-Puro

    Plasmid

    #207742

    Purpose

    Co-expression of the Cre recombinase and a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-Puro
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge