We narrowed to 4,489 results for: TIL;

1. GFP-Rock1-GBD (840-110 aa) in pEGFPN1

   Plasmid

   #187279

   Purpose

   Express EGFP-Rock1 GTPase-binding domain

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Rock1 (Rock1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   GBD (84-110aa)

   Promoter

   CMV

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIN2_mcm-3_mScarlet

   Plasmid

   #182933

   Purpose

   Expresses mcm-3 under its native promoter (embryonic nuclei and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

   Depositor

   Maureen Barr

   Article

   Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

   Insert

   genomic mcm-3 fused to mScarlet
   

   Tags

   flexible linker (GGGGS)x3 and mScarlet tag

   Expression

   Worm

   Promoter

   mcm-3

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVH666

   Plasmid

   #169610

   Purpose

   Tier-2 vector encoding PTtgO2-driven SEAP-p2A-iRFP670, PmPGK1-driven TtgA and PmPGK1-driven mRuby2 expression (PTtgO2-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-TtgA-pA::PmPGK1-mRuby2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression, PPGK-driven TtgA expression cassette and PPGK-driven YPet expression cassette
   

   Expression

   Mammalian

   Promoter

   tetO7-PCMVmin / PPGK / PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVH664

   Plasmid

   #169608

   Purpose

   Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven rtTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-rtTA-pA::PmPGK1-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression, PPGK-driven rtTA expression cassette and PPGK-driven YPet expression cassette
   

   Expression

   Mammalian

   Promoter

   tetO7-PCMVmin / PPGK / PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

   Plasmid

   #169597

   Purpose

   Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9 - VP64 fusion
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

   Plasmid

   #169665

   Purpose

   Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PhCMV / PPGK / RPBSA

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

   Plasmid

   #169664

   Purpose

   Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven YPet and PuroR expression
   

   Expression

   Mammalian

   Promoter

   PhCMV / RPBSA

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVH663

   Plasmid

   #169607

   Purpose

   Tier-2 vector encoding PTetO7-driven SEAP-p2A-iRFP670, PmPGK1-driven tTA and PmPGK1-driven YPet expression (PTetO7-CMVmin-2-SEAP-p2A-iRFP670-pA::PmPGK1-tTA-pA::PmPGK1-YPet-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP and iRFP expression, PPGK-driven tTA expression cassette and PPGK-driven YPet expression cassette
   

   Expression

   Mammalian

   Promoter

   tetO7-PCMVmin / PPGK / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH19-Tier1-PhCMV-p300core

   Plasmid

   #169572

   Purpose

   Tier-1 vector encoding PhCMV-driven histone acetyltransferase p300 core domain expression (PhCMV-p300core-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P300core (EP300 Human)
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTS2304-Tier1-Tactb-PPGK-NLuc_TB

    Plasmid

    #169549

    Purpose

    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    Tactb-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

    Plasmid

    #169537

    Purpose

    Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTS2380-Tier1-PhCMV-TM

    Plasmid

    #169485

    Purpose

    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) (PhCMV-TM-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven Junctophilin 2 transmembrane domain (JPH2 Human)
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pL-mWasabi

    Plasmid

    #176552

    Purpose

    Expression of mWasabi for auxotrophic selection in the absence of leucine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    mWasabi
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pM-eCFP

    Plasmid

    #176558

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of methionine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH-TagRFP657

    Plasmid

    #176560

    Purpose

    Expression of TagRFP657 for auxotrophic selection in the absence of lysine

    Depositor

    Markus Ralser

    Article

    Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

    Insert

    TagRFP657
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-hPPP3R2

    Plasmid

    #179163

    Purpose

    Expression vector of human protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, regulatory subunit B, beta isoform (PPP3R2 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-mPpp3r2

    Plasmid

    #179161

    Purpose

    Expression vector of mouse protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, regulatory subunit B, beta isoform (Ppp3r2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-mSpata33 (I81A)-PA

    Plasmid

    #179131

    Purpose

    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. I81A mutation is introduced in Spata33.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (Spata33 Mouse)
    

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Changed Isoleucine 81 to Alanine

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-hSPATA33-8xHIS-1D4

    Plasmid

    #179133

    Purpose

    Expression vector of human spermatogenesis associated 33 (SPATA33) tagged with 8XHIS and 1D4 at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (SPATA33 Human)
    

    Tags

    1D4 and 8XHIS

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-mSpata33 (P79A)-PA

    Plasmid

    #179130

    Purpose

    Expression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal. P79A mutation is introduced in Spata33.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    spermatogenesis associated 33 (Spata33 Mouse)
    

    Tags

    PA

    Expression

    Mammalian

    Mutation

    Changed Proline 79 to Alanine

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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