We narrowed to 73,554 results for: LAS

1. C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r

   Plasmid

   #162954

   Purpose

   Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Insert

   His6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

   Plasmid

   #149346

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbc

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

   Plasmid

   #149347

   Purpose

   Mammalian expression vector for SpyCas9 and puromycin resistance

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

   Plasmid

   #149350

   Purpose

   Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

   Depositor

   Ralf Kuehn

   Article

   Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

   Insert

   SpyCas9
   

   Use

   CRISPR

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Jan. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fast-GCaMP-RS09

   Plasmid

   #52649

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-RS09
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L414T mutated from GCaMP3

   Promoter

   T7

   Available since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

6. Fast-GCaMP-RS05

   Plasmid

   #52646

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-RS05
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M373Q mutated from GCaMP3

   Promoter

   T7

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

7. Fast-GCaMP-RS08

   Plasmid

   #52648

   Purpose

   genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

   Depositor

   Samuel Wang

   Article

   Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

   Insert

   Fast-GCaMP-RS08
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T412Q/L414T mutated from GCaMP3

   Promoter

   T7

   Available since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

8. UAS-Myc-dASUN

   Plasmid

   #47036

   Purpose

   Drosophila expression of tagged dASUN

   Depositor

   Laura Lee

   Article

   Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

   Insert

   Asunder (asun Fly)
   

   Use

   Tags

   Myc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

9. TV3-mCHY-dASUN

   Plasmid

   #47034

   Purpose

   Drosophila testes specific expression of fluorescent dASUN

   Depositor

   Laura Lee

   Article

   Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

   Insert

   Asunder (asun Fly)
   

   Use

   Tags

   mCHY

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. TV3-eGFP-dASUN

    Plasmid

    #47033

    Purpose

    Drosophila testes specific expression of fluorescent dASUN

    Depositor

    Laura Lee

    Article

    Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

    Insert

    Asunder (asun Fly)
    

    Use

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCR2.1-SNORD116-3

    Plasmid

    #45891

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Clin Med Insights Case Rep. 2013 May 5;6

    Insert

    SNORD115 with 80nt from intron sequence but without flanking exons (SNORD116-3 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pX330-U6-Chimeric_BB-CBh-hSpCas9

    Plasmid

    #42230

    Purpose

    A human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Has Service

    Cloning Grade DNA

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCH67 (crRNA expression vector)

    Plasmid

    #217337

    Purpose

    CROP-seq crRNA expression vector with GFP and puro marker

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    AsDR-filler-AsDR8, GFP-puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pdCas9-bacteria

    Plasmid

    #44249

    Purpose

    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dCas9 (bacteria)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    pLtetO-1

    Available since

    April 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-EGFP-CNA35

    Plasmid

    #61603

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with EGFP

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    EGFP and CNA35
    

    Use

    Tags

    His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRY2PHR-mCherryN1

    Plasmid

    #26866

    Purpose

    Expresses CRY2(1-498)-mCherry fusion for use in light-inducible protein interaction modules

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CRY2PHR (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMPMA3dPlac-PpipB-GFPLVA

    Plasmid

    #23344

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-TDsmURFP-IRES-HO-1

    Plasmid

    #80346

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and IRES

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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