We narrowed to 4,341 results for: TIL

1. pAAV_hSyn_WiChR_TS_mScarlet_ER

   Plasmid

   #205997

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of neurons

   Depositor

   Johannes Vierock, Peter Hegemann

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Wobblia Inhibitory Channelrhodopsin
   

   Use

   AAV

   Tags

   TS, mScarlet, ER

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hCathepsin B

   Plasmid

   #11249

   Purpose

   Mammalian expression of human cathepsin B

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2005 Dec 8. ():.

   Insert

   cathepsin B (CTSB Human)
   

   Use

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pYPQ-CBE-Cas9n-Act3.0

   Plasmid

   #178955

   Purpose

   It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP1

   Plasmid

   #79873

   Purpose

   Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

   Depositor

   Stanley Qi, Carol Gross, Jason Peters

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   xylA

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDG459

   Plasmid

   #100901

   Purpose

   SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

   Depositor

   Paul Thomas

   Article

   Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

   Inserts

   humanized CRISPR associated protein 9
   U6-gRNA scaffold 1
   U6-gRNA scaffold 2
   

   Use

   CRISPR and Mouse Targeting

   Tags

   3xFLAG and T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK170.AAV-TRE-Cre-WPRE (Supernova)

   Plasmid

   #85040

   Purpose

   For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nlsCre-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. ROS lux sensor

   Plasmid

   #199791

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

   Insert

   ROS lux Sensor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ-ABE-Cas9n-Act3.0

   Plasmid

   #178956

   Purpose

   It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-Dual-iCas9-CMV

   Plasmid

   #167500

   Purpose

   Expression of dual inducible Cas9 with CMV promoter

   Depositor

   Jake Liang

   Article

   Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   Cas9
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLL5.0-MRLC-DD-GFP

    Plasmid

    #187260

    Purpose

    Lentiviral expression of myosin regulatory light chain-DD mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    T18D, S19D

    Promoter

    5' LTR

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLL5.0-MRLC-AA-GFP

    Plasmid

    #187259

    Purpose

    Lentiviral expression of myosin regulatory light chain-AA mutant

    Depositor

    Alpha Yap

    Article

    Wee et al J Cell Sci. 2020 Jul 6;133(13). pii: jcs

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    T18A,S19A

    Promoter

    5' LTR

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

    Plasmid

    #122084

    Purpose

    Express M.EcoGII fused to TRF1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-TERF1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of TERF1

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLM1110_LEU2

    Plasmid

    #168460

    Purpose

    Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

    Depositor

    Jef Boeke

    Article

    Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

    Insert

    mRFP1
    

    Use

    Synthetic Biology; Yeast assembly vector for big-…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-Dual-iCas9-EFS

    Plasmid

    #167501

    Purpose

    Expression of dual inducible Cas9 with EFS promoter

    Depositor

    Jake Liang

    Article

    Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    Cas9
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CKIIa-stGtACR2-FusionRed

    Plasmid

    #105669

    Purpose

    Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde and AAV1

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CKIIa-stGtACR1-FusionRed

    Plasmid

    #105679

    Purpose

    Expresses a soma-targeted GtACR1 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Insert

    stGtACR1
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG mEmerald-EB3

    Plasmid

    #168514

    Purpose

    Expression of human plus-TIP microtubule binding protein EB3 fused with mEmerald for microtubule +end live tracking

    Depositor

    Franck Polleux

    Article

    Lewis TL et al Curr Biol. 2016 Oct 10;26(19):2602-2608.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB19

    Plasmid

    #194757

    Purpose

    EGFP-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    EGFP-Tag

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCB20

    Plasmid

    #194758

    Purpose

    ALFA-tagged GluRIIA-Construct for integration at attP sites

    Depositor

    Sven Dannhäuser

    Article

    Beckers et al Cells. 2024 Feb 10;13(4):323. doi: 10.33

    Insert

    GluRIIA (GluRIIA Fly)
    

    Use

    Phic31-integration vector

    Tags

    ALFA-Tag

    Expression

    Mutation

    none

    Promoter

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3.1_WiChR_TS_mScarlet_ER

    Plasmid

    #195190

    Purpose

    High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells

    Depositor

    Johannes Vierock, Peter Hegemann

    Article

    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

    Insert

    Wobblia lunata inhibitory Channelrhodopsin
    

    Use

    Tags

    ER-export sequence, Kir2.1 trafficking motif, and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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