We narrowed to 6,905 results for: plasmid dna

1. pL0_AC-Fld

   Plasmid

   #202125

   Purpose

   Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   Fld promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter sequence mutated to remove bsaI site for…

   Promoter

   Available Since

   Aug. 24, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pL0_AC-54730

   Plasmid

   #202109

   Purpose

   Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   54730 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter sequence mutated to remove bsaI site for…

   Promoter

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pL0_AC-51183

   Plasmid

   #202108

   Purpose

   Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   51183 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter sequence mutated to remove sapI sites fo…

   Promoter

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pL0_AC-GEF

   Plasmid

   #202107

   Purpose

   Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   GEF promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter sequence mutated to remove sapI site for…

   Promoter

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pL0_AC-47740

   Plasmid

   #202106

   Purpose

   Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   47740 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter sequence mutated to remove bsaI site for…

   Promoter

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pL0_AC-BCA

   Plasmid

   #202105

   Purpose

   Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   BCA promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter sequence mutated to remove sapI sites fo…

   Promoter

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pL0_AC-epyC

   Plasmid

   #202104

   Purpose

   Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Insert

   epyC promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter sequence mutated to remove bsaI sites fo…

   Promoter

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pSCL.040

   Plasmid

   #184974

   Purpose

   Test effect of extending a1/a2 on ADE2 editing rates in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   ADE2 donor P272stop, a1/a2 length extended to 27 …

   Promoter

   Gal7

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSCL.109

   Plasmid

   #184978

   Purpose

   Test effect of extending a1/a2 on LYP1 editing rates in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   LYP1 donor E27stop, a1/a2 length extended to 27 bp

   Promoter

   Gal7

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSCL.107

    Plasmid

    #184976

    Purpose

    Test effect of extending a1/a2 on CAN1 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    CAN1 donor G444stop, a1/a2 length extended to 27 …

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSCL.111

    Plasmid

    #184980

    Purpose

    Test effect of extending a1/a2 on TRP2 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TRP2 donor E64stop, a1/a2 length extended to 27 bp

    Promoter

    Gal7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSCL.113

    Plasmid

    #184982

    Purpose

    Test effect of extending a1/a2 on FAA1 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    FAA1 donor P233stop, a1/a2 length extended to 27 …

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCL.039

    Plasmid

    #184973

    Purpose

    Test effect of extending a1/a2 on ADE2 editing rates in yeast

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ADE2 donor P272stop, a1/a2 length extended to 27 …

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCSDest2-2XNLS-SpCas9-MT3-NLS-3XHA-NLS-TALentry

    Plasmid

    #69233

    Purpose

    R1335K mutant SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

    Expression

    Mammalian

    Mutation

    Arginine 1335 is changed to Lysine

    Promoter

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AP303-4

    Plasmid

    #66088

    Purpose

    co-expression of Cas9 and a sgRNA targeting 5'end of C.elegans K08F4.2

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APs1
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. AP334-5

    Plasmid

    #66096

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APs5
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. APq5271

    Plasmid

    #66085

    Purpose

    co-expression of Cas9 and a sgRNA targeting K08F4.2 in the middle of the ORF

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APa4-2
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. APCSD 54

    Plasmid

    #66100

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for CSD 54
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

19. APCSD 53

    Plasmid

    #66099

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans swan-2

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for CSD 53
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

20. AP334-6

    Plasmid

    #66097

    Purpose

    co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans K08F4.2

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

    Insert

    sgRNA for APs5
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available Since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only