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Showing: 451 - 480 of 8842 results

  1. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid
    #218865
    Purpose
    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Article
    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.
    Insert
    RSET-jGCaMP8s
    Tags
    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…
    Expression
    Insect
    Mutation
    S26M F286Y Q315H
    Promoter
    hsp70
    Available Since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218865-plasmid-map-sequence-id-434255

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  2. B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

    Plasmid
    #100716
    Purpose
    B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)
    Depositor
    Alberto Ciccia
    Article
    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17
    Insert
    sgSTOP targeting ATP1A1 (cloned using BsmBI)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 promoters
    Available Since
    Sept. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    100716-plasmid-map-sequence-id-193652

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  3. pJEP323-pAAV-FullH1TO-SaCa9gRNAi(SapI)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113700
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in a gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    113700-plasmid-map-sequence-id-231558

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  4. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available Since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  24. UbiGate Components (set1)

    Plasmid Kit
    #1000000144
    Purpose
    Generate bacterial expression cassettes of up to six ORFs simultaneously; also contains ubiquitin, the E1 UBA1, an E2 collection, and a selection of E3s and their substrates for ubiquitination assays
    Depositor
    Marco Trujillo
    Available Since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  25. EasyCloneYALI- CRISPR/Cas9 Set

    Plasmid Kit
    #1000000141
    Purpose
    Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9
    Depositor
    Irina Borodina
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  26. EasyClone-MarkerFree Vector Set

    Plasmid Kit
    #1000000098
    Purpose
    Yeast (S. cerevisiae) vector toolkit for marker-less integration of genes into 11 predetermined EasyClone Chromosomal loci via CRISPR-Cas9
    Depositor
    Irina Borodina
    Available Since
    Dec. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. EasyCloneYALI- Integrative Vector Set

    Plasmid Kit
    #1000000140
    Purpose
    Contains integrative plasmids suitable for (over-)expression of (heterologous) genes in Y. lipolytica strains
    Depositor
    Irina Borodina
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  28. UbiGate E2 activity screen (set2)

    Plasmid Kit
    #1000000145
    Purpose
    Contains bacterial constructs for E3 autoubiquitination assay, including expression cassettes containing ubiquitin and the E1 UBA1 in combination with all Arabidopsis thaliana E2s
    Depositor
    Marco Trujillo
    Available Since
    Feb. 1, 2019
    Availability
    Academic Institutions and Nonprofits only

  29. Endy/Shearwin pOSIP plasmid set

    Plasmid Kit
    #1000000035
    Purpose
    11 Plasmids for use in clonetegration: method for decreasing time and effort needed for integration of DNA into prokaryotic chromosomes. Facilitates cloning, expression of difficult genetic elements
    Depositor
    Drew Endy
    Available Since
    July 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. Target Accelerator Genetic Pathway Reference Set

    Plasmid Kit
    #1000000105
    Purpose
    Collection of genes and mutations for reference pathway signatures and phenotypes to compare with those derived from cancer alleles; represent over 25 cellular signaling pathways and processes
    Depositor
    Jesse Boehm
    Available Since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 451 - 480 of 8842 results