We narrowed to 20,206 results for: INO
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Plasmid#149395PurposeP. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationIHFprox site is optimized at key positions needed…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_motif_scram
Plasmid#149397PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationUpstream motif recognized by Cas1-2/3 during spac…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha N90F
Plasmid#58257Purposebacterial expression of human PITP alpha N90FDepositorInsertPITP alpha (PITPNA Human)
TagsHISExpressionBacterialMutationN90F (abolishes PI binding)PromoterT7Available SinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET14b-PITPalpha K61A
Plasmid#58258Purposebacterial expression of human PITP alpha K61ADepositorInsertPITP alpha (PITPNA Human)
TagsHISExpressionBacterialMutationK61A (abolishes PI binding)PromoterT7Available SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Tags6XHisExpressionBacterialMutationnonePromoterT7Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HyPer-DAAO-NES
Plasmid#119164PurposeFusion of hydrogen peroxide fluorescent biosensor HyPer and D-amino acid oxidase; excluded from nucleus. Allows H2O2 production/detection by adding D-amino acids. AAV expression vector; CMV promoter.DepositorInsertHyPer-DAAO-NES
UseAAVTagsNESExpressionMammalianPromoterCMVAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-14 (LA-14)
Plasmid#158750PurposeLentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.DepositorInsertLifeAct-14
UseLentiviralTagseGFPExpressionMammalianMutationDeletion mutation for last three amino acids from…PromoterCMV immediate earlyAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS_v2
Plasmid#196485PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
ExpressionMammalianPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available SinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
TagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcpAzpaRS_v2
Plasmid#196483PurposeTo express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.DepositorInsertazidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
ExpressionMammalianPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG068
Plasmid#214259PurposepJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaIDepositorInsertCore genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
ExpressionBacterialMutation18 nucleotide 5' of the start codon (Shine-D…PromoteraraBAD promoterAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG059
Plasmid#214262PurposePaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)DepositorInserttLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
Tags6x His-tag and 6xHis-tagExpressionBacterialPromoterT7 promoterAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+7U
Plasmid#149393PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 7bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation7bp inserted upstream, and 10bp inserted downstre…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_+10D+10U
Plasmid#149394PurposeP. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream,and 10bp addition upstream of IHFprox siteDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutation10bp inserted upstream and downstream of the IHFp…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHF_Mut
Plasmid#149396PurposeP. aeruginosa PA14 CRISPR2 locus, with a mutated IHFprox site, that should not be recognized by IHFDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationIHFprox site is mutated at key positions needed f…PromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC_N168C His
Plasmid#173809PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Tags6XHisExpressionBacterialMutationAsn168Cys for labelingPromoterT7Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINTphiC31
Plasmid#127518PurposePlasmid encodes H. sapiens codon optimized Integrase phiC31.DepositorInsertIntegrase phiC31 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pUB-HspINTBxb1
Plasmid#127519PurposePlasmid encodes H. sapiens codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for H. sapiens expression.
ExpressionMammalianAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUAST attB Orco (Y478A)
Plasmid#73928PurposeUAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid changeDepositorAvailable SinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET20b_Pa-PiuA
Plasmid#128955Purposeexpresses Pseudomonas aeruginosa PiuA in E. coliDepositorInsertiron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
Tags6xHis and pelB leaderExpressionBacterialMutationSignal peptide sequence has been removedPromoterT7Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only