We narrowed to 21,553 results for: INO

1. EGFP-UTRN-mini

   Plasmid

   #158749

   Purpose

   Expresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cells

   Depositor

   Minhajuddin Sirajuddin

   Article

   Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

   Insert

   UTRN-mini
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR2_+1D

   Plasmid

   #149389

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 1bp addition downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   1bp inserted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSG069

   Plasmid

   #214260

   Purpose

   pJN105 with PaftsH2SD and PaFtsH2 ORF cloned between NheI/XbaI

   Depositor

   Ute Romling

   Article

   Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

   Insert

   tLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD promoter

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPR2_IRdist_scram

   Plasmid

   #162319

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   The distal Upstream Motif site is scrambled

   Promoter

   pBAD

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA424::tsi5

   Plasmid

   #192960

   Purpose

   Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

   Depositor

   David Albesa-Jové

   Article

   Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

   Insert

   tsi5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR2_-6D

   Plasmid

   #149387

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   6bp deleted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR2_-5D

   Plasmid

   #149388

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   5bp deleted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCRISPR2_+5D

   Plasmid

   #149390

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   5bp inserted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPR2_+10D

   Plasmid

   #149391

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with 10bp addition downstream of IHFprox site

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   10bp inserted downstream of the IHFprox site

   Promoter

   pBAD

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR2_IHF_Opt

    Plasmid

    #149395

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with "optimized" IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    IHFprox site is optimized at key positions needed…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR2_motif_scram

    Plasmid

    #149397

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with a scrambled Upstream recognition motif

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Upstream motif recognized by Cas1-2/3 during spac…

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET14b-PITPalpha N90F

    Plasmid

    #58257

    Purpose

    bacterial expression of human PITP alpha N90F

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    N90F (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET14b-PITPalpha K61A

    Plasmid

    #58258

    Purpose

    bacterial expression of human PITP alpha K61A

    Depositor

    Shamshad Cockcroft

    Article

    Tilley et al Structure. 2004 Feb;12(2):317-26.

    Insert

    PITP alpha (PITPNA Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    K61A (abolishes PI binding)

    Promoter

    T7

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

14. LifeAct-14 (LA-14)

    Plasmid

    #158750

    Purpose

    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

    Insert

    LifeAct-14
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Deletion mutation for last three amino acids from…

    Promoter

    CMV immediate early

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-20b-M-Ntx-S1

    Plasmid

    #58716

    Purpose

    Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

    Depositor

    Fiorella Tonello

    Article

    Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

    Insert

    Notexin
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Changed Asparagine 1 to Serine

    Promoter

    T7

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBtPIPLC His

    Plasmid

    #173808

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcpBpaRS_v2

    Plasmid

    #196485

    Purpose

    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40(synthetase), Pol III tRNA promoter (tRNA), C…

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcpAzpaRS_v2

    Plasmid

    #196483

    Purpose

    To express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.

    Depositor

    Kensaku Sakamoto

    Article

    Hino et al Methods Mol Biol. 2012;794:215-28. doi:

    Insert

    azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40(synthetase), Pol III tRNA promoter (tRNA), C…

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSG068

    Plasmid

    #214259

    Purpose

    pJN105 with PaftsH2SD and PaFtsH1 ORF cloned between NheI/XbaI

    Depositor

    Ute Romling

    Article

    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

    Insert

    Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    18 nucleotide 5' of the start codon (Shine-D…

    Promoter

    araBAD promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSG059

    Plasmid

    #214262

    Purpose

    PaFtsH2-6xHis cloned in XbaI/NheI site in pET-28a(+)

    Depositor

    Ute Romling

    Article

    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

    Insert

    tLST accessory element PaFtsH2 protease of Pseudomonas aeruginosa SG17M
    

    Use

    Tags

    6x His-tag and 6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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