We narrowed to 2,420 results for: NIG

1. TetO-Ascl1-puro

   Plasmid

   #97329

   Purpose

   Generating lentiviruses to express Ascl1 and puromycin resistance

   Depositor

   Marius Wernig

   Article

   Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TET-ON

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTet-O-Nd1-puro

   Plasmid

   #52052

   Purpose

   Expresses Nd1 and puromycin resistent gene under control of TetON promoter

   Depositor

   Marius Wernig

   Article

   Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

   Insert

   Neurod1 (Neurod1 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A-and puromycin resistant cassette

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV[Exp]-Neo-EF1A>Tet3G

   Plasmid

   #184379

   Purpose

   The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Tet3G
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV[Tet]-Puro-TRE3G>mMyod1[NM_010866.2]

   Plasmid

   #184380

   Purpose

   The mouse Myod1 gene is expressed under a doxcycline-inducible TRE3G promoter

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Myod1 (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJV126

   Plasmid

   #124250

   Purpose

   In vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJV127

   Plasmid

   #124251

   Purpose

   In vitro transcription plasmid for T7 transcription of Her2 ITD sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJV128

   Plasmid

   #124252

   Purpose

   In vitro transcription plasmid for T7 transcription of KRAS WT sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJV129

   Plasmid

   #124253

   Purpose

   In vitro transcription plasmid for T7 transcription of KRAS G12V sequence targeted to the endosome. Linearize with SapI.

   Depositor

   Stanley Riddell

   Article

   Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

   Insert

   Minigene containing KRAS sequences fused to endosomal targeting sequence of class I MHC
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWAP-HGF

   Plasmid

   #83503

   Purpose

   Generation of transgenic mice overexpressing the HGF under the control of the WAP gene promoter

   Depositor

   Marta Gallego, Lothar Hennighausen

   Article

   Gallego et al Oncogene. 2003 Nov 20;22(52):8498-508.

   Insert

   HGF (Hgf Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   also contains beta-globin sequences from pUC198

   Promoter

   mouse WAP (whey acidic protein)

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIV313

    Plasmid

    #204956

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7, hph (hygromycin) resistance marker and sgRNA targeting albA locus in A. niger

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    hph (hygromycin resistance marker)
    sgRNA (CAGCAATGCTTCCATGCAATT) targeting albA locus in A. niger flanked by a tRNA-Gly repeat
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus fumigatus U3 promoter, Aspergillus ni…

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

    Plasmid

    #184381

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Cas9, two gRNAs targeting dystrophin
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. rd

    Plasmid

    #112912

    Purpose

    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag

    Depositor

    C. James McKnight

    Article

    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

    Insert

    dematin (DMTN Human)
    

    Use

    Tags

    Glutatione-S-Tranferase and precision protease si…

    Expression

    Bacterial

    Mutation

    PEST sequence near the N-terminal removed. 5′-GCG…

    Promoter

    T7

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-EF1a-MICB*005-IRES-ZsGreen

    Plasmid

    #114008

    Purpose

    Induces expression of human MICB allele 005 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICB*005 (MICB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-EF1a MICA*009-IRES-ZsGreen

    Plasmid

    #114007

    Purpose

    Induces expression of human MICA allele 009 cDNA

    Depositor

    Kai Wucherpfennig

    Article

    Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

    Insert

    MICA*009 (MICA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. rd-his

    Plasmid

    #112911

    Purpose

    Express the PEST domain-replaced 48 kDa form of human dematin with a cleavable N-terminal GST tag and non-cleavable C-terminal 6-his tag

    Depositor

    C. James McKnight

    Article

    Chen et al J Biol Chem. 2013 Mar 22;288(12):8313-20

    Insert

    dematin (DMTN Human)
    

    Use

    Tags

    6-his tag, Glutathione-S-Transferase, and preciss…

    Expression

    Bacterial

    Mutation

    89KSTSPPPSPEVWAD102 was replaced with 89KAAAGGGAG…

    Promoter

    T7

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTet-O-Ngn2-puro

    Plasmid

    #52047

    Purpose

    3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

    Depositor

    Marius Wernig

    Article

    Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

    Insert

    Ngn2 (Neurog2 Mouse)
    

    Use

    Lentiviral

    Tags

    T2A-and puromycin resistant cassette

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on

    Available since

    April 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

    Plasmid

    #176484

    Purpose

    TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PiggyBac-TetO-En1-t2a-Foxa2-hygro

    Plasmid

    #176483

    Purpose

    TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    En1-t2a-FoxA2 (FOXA2, En1 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-dCas9-p300-P2A-PuroR

    Plasmid

    #83889

    Purpose

    Lentiviral Sp dCas9-p300-P2A-PuroR

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    pac from Streptomyces alboniger
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EFS

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCag FlpO-2A-Cre EV

    Plasmid

    #129419

    Purpose

    episomal expression of FlpO and Cre recombinases

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    FlpO-2A-Cre
    

    Use

    Cre/Lox and Unspecified; Episomal expression vect…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/Chick β-actin (CAG)

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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