-
Plasmid#50886PurposeExpresses human NKCC1 W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationW732C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 L671C (NT440)
Plasmid#50872PurposeExpresses human NKCC1 L671C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationL671C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 E670C (NT473)
Plasmid#50871PurposeExpresses human NKCC1 E670C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
UseTags3x Flag and YFPExpressionMammalianMutationE670C in hNKCC1 in NT17PromoterCMVAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-iCre
Plasmid#89573PurposeExpresses iCre under pCAGDepositorInsertiCre
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pThpR
Plasmid#190613PurposeExpresses GFP in response to tetrahydropapaverine in E. coliDepositorInsertTHP4.1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pS448∙CsR
Plasmid#122096PurposeCRISPR/Cas9 counterselection in Gram-negative bacteriaDepositorInsertsCas9
sgRNA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPem7 and PmAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-VCre
Plasmid#89575PurposeExpresses VCre under pCAGDepositorInsertVCre recombinase
UseCre/Lox and Synthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RILP
Plasmid#74600Purposeused for expression in E. coli and purificationDepositorInsertRab-interacting lysosomal protein (RILP Human)
UseTagsFLAG, StrepII, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pReg-RamR
Plasmid#190610PurposeExpresses the RamR transcription factor in E. coliDepositorInsertRamR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pramr-GFP
Plasmid#194148PurposeContains a RamR-regulated promoter expressing GFPDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterPramrAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
SRpHi4
Plasmid#102544PurposeBacterial expression of EYFP fused to Epidermal Growth Factor with a GST tag for purificationDepositorInsertEYFP-EGF
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO C1-APEX2
Plasmid#129641PurposeExpress ER-localized C1-APEX2 fusion in mammalian cellsDepositorInsertC1(1-29)-APEX2-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
SRpHi2
Plasmid#102542PurposeBacterial expression of EGFP fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-EGFP
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
SRpHi3
Plasmid#102543PurposeBacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-sepHluorin
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
SRpHi1
Plasmid#102541PurposeBacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-EYFP
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MAVS-M142A(KaganF61)
Plasmid#51999PurposeExpresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"DepositorInsertMAVS
UseTagsHAExpressionMammalianMutationMet 142 to AlaPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3XFlag-CMV7-miniMAVS(KaganE48)
Plasmid#52019PurposeExpresses miniMAVS (the sequence starting from M142 of the MAVS CDS) with an N-terminal 3X flag tagDepositorInsertminiMAVS
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pReg-ROTU4
Plasmid#190669PurposeExpresses an evolved rotundine biosensor in E. coliDepositorInsertROTU4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pReg-THP4
Plasmid#190671PurposeExpresses an evolved tetrahydropapaverine biosensor in E. coliDepositorInsertTHP4
UseSynthetic BiologyTagsExpressionMutationPromoterJ23103Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGfOMT1-GEN4
Plasmid#190612PurposeExpresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coliDepositorInsertGfOMT-GEN4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only