We narrowed to 17,687 results for: URE

1. dxCas(3.6)-VPR

   Plasmid

   #108388

   Purpose

   Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   dxCas(3.6)-VPR
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Promoter

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEos2-Actin-7

   Plasmid

   #57339

   Purpose

   Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Actin (ACTB Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

3. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. VVT1

   Plasmid

   #65779

   Purpose

   The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   SaCas9 gRNA backbone, without spacer sequence
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. [pSK475] pLJC5 HA-Depdc5(A)

   Plasmid

   #109344

   Purpose

   Lenti-viral expression of HA-tagged Depdc5 mutant A

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   DIYGD(185-189)GSGSG

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. [pSK476] pLJC5 HA-Depdc5(B)

   Plasmid

   #109345

   Purpose

   Lenti-viral expression of HA-tagged Depdc5 mutant B

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   EQPLH(371-375)GSGSG

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. [pSK478] pLJC5 HA-Depdc5(P)

   Plasmid

   #109347

   Purpose

   Lenti-viral expression of HA-tagged Depdc5 mutant P

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   YDLLP(775-779)GSGSG

   Promoter

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEos2-Talin-IBS2-7

   Plasmid

   #57424

   Purpose

   Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Article

   Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

   Insert

   Talin IBS2 (Tln1 Mouse)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Talin Integrin Binding Site

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pSmBtA3-3AF

   Plasmid

   #245785

   Purpose

   Expression of mutated bovine arrestin-3 (I385A, V386A, F387A) tagged with SmBiT and Flag in mammalian cells

   Depositor

   Vsevolod Gurevich

   Article

   Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

   Insert

   arrestin-3 (ARR3 Bovine)
   

   Use

   Tags

   Flag and SmBiT

   Expression

   Mammalian

   Mutation

   I385A, V386A, F387A

   Promoter

   CMV

   Available Since

   Oct. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVeBtA3

    Plasmid

    #238988

    Purpose

    Mammalian expression of bovine arrestin-3 short isoform with Venus N-terminal fusion

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

    Insert

    arrestin-3 short (ARR3 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVeBtA2

    Plasmid

    #238987

    Purpose

    Mammalian expression of bovine arrestin-2 long isoform with Venus N-terminal fusion

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

    Insert

    arrestin-2 long (ARRB2 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVeBtA2-3A

    Plasmid

    #238991

    Purpose

    Mammalian expression of mutant bovine arrestin-2 long isoform with Venus N-terminal fusion

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Mar 16;287(12):9028-40

    Insert

    arrestin-2 long (ARRB2 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    I386A,V387A,F388A

    Promoter

    CMV

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. BtA3CL

    Plasmid

    #238992

    Purpose

    In vitro transcription of mutant bovine arrestin-3 short isoform

    Depositor

    Vsevolod Gurevich

    Article

    Ahmed et al Biochemistry. 2011 May 10;50(18):3749-63

    Insert

    arrestin-3 short (ARR3 Bovine)
    

    Use

    In vitro transcription

    Tags

    Expression

    Mutation

    C17S, C60 V, C126S, C141L, C151V, C243V, C252V, C…

    Promoter

    SP6

    Available Since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVEA3-delta7

    Plasmid

    #234802

    Purpose

    Mammalian expression of bovine arrestin-3 (deleted residues 176, 177, 178, 182-185)

    Depositor

    Vsevolod Gurevich

    Article

    Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    deleted residues 176, 177, 178, 182-185

    Promoter

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. p3VEA3-3A

    Plasmid

    #234797

    Purpose

    Mammalian expression of bovine arrestin-3 (I385A,V386A,F387A)

    Depositor

    Vsevolod Gurevich

    Article

    Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

    Insert

    Arrestin-3 (ARR3 Bovine)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    I385A,V386A,F387A

    Promoter

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. ReaLiSM A1-26link-GPIba+2cys

    Plasmid

    #59580

    Purpose

    express VWF A1-GPIba ReaLiSM construct in mammalian cells

    Depositor

    Timothy Springer

    Article

    Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

    Insert

    VWF A1-linker-GPIba (VWF Human)
    

    Use

    Tags

    6His

    Expression

    Mammalian

    Mutation

    Promoter

    chick beta-actin

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

17. P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

    Plasmid

    #239972

    Purpose

    Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

    Plasmid

    #239974

    Purpose

    PiggyBac plasmid for inducible PEmax expression and Puromycin selection

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid

    #183790

    Purpose

    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Inserts

    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    G>A in the gRNA scaffold- please see depositor…

    Promoter

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Rlucrarr3-Flash5

    Plasmid

    #74133

    Purpose

    FlAsH-BRET reporter to monitor conformational changes in Arrestin3. Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 of Arrestin.

    Depositor

    Louis Luttrell

    Article

    Lee et al Nature. 2016 Mar 31;531(7596):665-8. doi

    Insert

    Arrestin3
    

    Use

    Tags

    Renilla luciferase (rLuc)

    Expression

    Mammalian

    Mutation

    Insertion of FlAsH motif (CCPGCC) following amino…

    Promoter

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only