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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,337 results for: chm

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Showing: 301 - 320 of 1337 results

  1. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid
    #196712
    Purpose
    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3
    Depositor
    Bernhard Schmierer
    Insert
    dCas9-KRAB(ZIM3)-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    196712-plasmid-map-sequence-id-388051

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  3. pHBintE

    Plasmid
    #48136
    Purpose
    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector
    Depositor
    Dieter Jahn
    Publication
    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Oct. 21, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    48136-plasmid-map-sequence-id-69942

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  4. lenti.Cas9.BFP.Blast

    Plasmid
    #196714
    Purpose
    WT SpCas9 with BSD-TagBFP for selection and sorting
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    196714-plasmid-map-sequence-id-388043

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  5. pEF-XMAS-1xStop

    Plasmid
    #164411
    Purpose
    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.
    Depositor
    Xiao Wang
    Publication
    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Tags
    T2A-GFP
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    164411-plasmid-map-sequence-id-337634

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  7. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid
    #196727
    Purpose
    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    TRE
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196727-plasmid-map-sequence-id-389689

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  12. TNFR2-GFP

    Plasmid
    #111207
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TNFRSF1B (TNFRSF1B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S175P, D306G
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111207-plasmid-map-sequence-id-213090

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  13. EGFP-AR

    Plasmid
    #111215
    Purpose
    fluorescent fusion protein
    Depositor
    Johannes A. Schmid
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AR (Ar Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111215-plasmid-map-sequence-id-264404

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  15. mCherry-APP-mGFP

    Plasmid
    #196704
    Purpose
    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    mCherry and mEGFP
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196704-plasmid-map-sequence-id-386429

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  16. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    109428-plasmid-map-sequence-id-212894

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  18. pMBP-SpyCatcher

    Plasmid
    #79053
    Purpose
    Expresses maltose binding protein (MBP) SpyCatcher fusion
    Depositor
    James Bowie
    Publication
    Min et al Protein Sci. 2016 Aug;25(8):1535-44. doi
    Insert
    MBP-SpyCatcher
    Tags
    6xHis and Maltose binding protein
    Expression
    Bacterial
    Mutation
    Derived from pDEST14-SpyCatcher (Plasmid #35044)…
    Promoter
    T7
    Available since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    79053-plasmid-map-sequence-id-147896

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  19. pcDNA3-MTS-KD-c-Src-FLAG

    Plasmid
    #44653
    Depositor
    Yoshimi Homma
    Publication
    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    Tags
    FLAG tag and mitochondria-targeting sequence (MTS…
    Expression
    Mammalian
    Mutation
    changed lysine 298 to methionine
    Promoter
    CMV promoter
    Available since
    May 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    44653-plasmid-map-sequence-id-61243

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Showing: 301 - 320 of 1337 results