Addgene

We narrowed to 2,456 results for: gal.1

Showing: 301 - 320 of 2456 results

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CRY-GalVP16l (B694)

Plasmid

#92030

Purpose

expresses CRY2 (Full length, mutant NLS) fused to Gal4DNA (residues 1-147) fused to VP16 activation domain (long form)

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

GalBD-VP16

Expression

Mammalian

Mutation

NLS sequence of CRY2 mutated

Promoter

CMV

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

Plasmid

#107739

Purpose

mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein(C1A)

Use

Tags

Expression

Worm

Mutation

change the first cysteine of gp41-1 N-intein to a…

Promoter

C. elegans myo-2 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTS2374-Tier1-PhCMV-Gal4_VP16

Plasmid

#169582

Purpose

Tier-1 vector encoding PhCMV-driven Gal4-VP16 transactivator (PhCMV-Gal4-VP16-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

GAL4-transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAG413 GAL ORF1 Shuffle1 NLS

Plasmid

#145794

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Payero et al Journal of the South Carolina Academy of

Insert

ORF1 Shuffle1 NLS

Use

Tags

Expression

Yeast

Mutation

Enhanced Nucleur Localization Signal

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-LGALS8

Plasmid

#175758

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS8 (LGALS8 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426 Gal TDP43 GFP

Plasmid

#27467

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-p53

Plasmid

#171076

Purpose

Expresses Gal4-p53 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Use

Tags

His and p53(1-85)

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pRS303 Gal TDP-43 YFP

Plasmid

#27468

Depositor

Aaron Gitler

Article

Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

Insert

TDP-43 (TARDBP Human)

Use

Tags

YFP

Expression

Yeast

Mutation

D23A mutation in TDP-43 sequence

Promoter

Available since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only
pET-Galectin-8 linker deletion

Plasmid

#68806

Purpose

Express Galectin-8 linker deletion protein in Bacterial cells

Depositor

Hyun Kyu Song

Article

Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/nc

Insert

Galectin-8 (LGALS8 Human)

Use

Tags

N-terminal 6-histidine-tag

Expression

Bacterial

Mutation

No mutation

Promoter

T7

Available since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only
pEJS887_pTetR-P2A-BFPnls/sgAlpha

Plasmid

#108651

Purpose

Alpha satellite repeats targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Alpha satellite repeats targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Mutation

Promoter

U6 and hPGK

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-GALK1

Plasmid

#23743

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GALK1 (GALK1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
GAL-hAR-658-919

Plasmid

#89082

Purpose

mammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

Insert

hAR-658-919 (AR Human)

Use

Tags

GAL4 DBD

Expression

Mammalian

Mutation

aa 658-919

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S133A

Plasmid

#46770

Purpose

Expression of GAL4 fusion to CREB-S133A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S133A (Creb1, GAL4 Rat, Budding Yeast)

Use

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 133 to Alanine

Promoter

CMV

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S142A

Plasmid

#46771

Purpose

Expression of GAL4 fusion to CREB-S142A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S142A (Creb1, GAL4 Rat, Budding Yeast)

Use

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 142 to Alanine

Promoter

CMV

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRS416 Gal-RNQ1 L94A-YFP

Plasmid

#18692

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 L94A (RNQ1 Budding Yeast)

Use

Tags

YFP

Expression

Yeast

Mutation

Leucine 94 mutated to Alanine (L94A)

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
Gal4-HA-HIF1α(530-652)

Plasmid

#24887

Depositor

William Kaelin

Article

Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

Insert

hypoxia inducible factor1alpha (HIF1A Human)

Use

Tags

Gal4dbd and HA-tag

Expression

Mammalian

Mutation

partial HIF1alpha cDNA (encoding aa 530-652)fused…

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPERFECT_shuffled1

Plasmid

#215577

Purpose

For overexpression of Gal4-DBD HOXD4 AroPERFECT shuffled 1

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPERFECT shuffled1 (HOXD4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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PC3-709-33-10_GAL4-FKBP12(F36V)

Plasmid

#200438

Purpose

Expresses the GAL4 DNA Binding domain linked to the FKBP12(F36V) target protein

Depositor

Xue Gao

Article

Ma et al J Am Chem Soc. 2023 Jan 25;145(3):1593-1

Insert

GAL4-FKBP12(F36V)

Use

Tags

GAL4, FKBP12(F36V)

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

Plasmid

#62953

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only
pRS416 Gal-RNQ1 153-405-YFP

Plasmid

#18689

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 153-405

Use

Tags

YFP

Expression

Yeast

Mutation

Prion domain of Rnq1

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only