We narrowed to 2,567 results for: gem

1. pGEMHE-mEGFP-mTrim21ΔRING-Box

   Plasmid

   #105520

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9_hGEM(1-110)_hSLBP(56-108)

   Plasmid

   #191388

   Purpose

   SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_hSLBP(56_108)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. KM-CashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174838

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   TEF1p

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMT-Map7-1333-end

   Plasmid

   #46070

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-1333-end (Map7 Mouse)
   

   Expression

   Bacterial

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLeo-A-a2-GEMS(JAK-STAT)

   Plasmid

   #209110

   Purpose

   Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   A-a2-GEMS(JAK-STAT)
   

   Expression

   Mammalian

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM-sgMUC4_dual-MTS-3x-Set I

   Plasmid

   #162762

   Purpose

   Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-and1 in situ probe

   Plasmid

   #52102

   Purpose

   actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

   Depositor

   Didier Stainier

   Article

   Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

   Insert

   actinodin 1 in situ probe (and1 Zebrafish)
   

   Expression

   Bacterial

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM-HE-Venus-bPAC(F198Y)

   Plasmid

   #165487

   Purpose

   Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   Venus-bPAC(F198Y)-myc
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Myc and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-T Easy-Gbait-hs-QF2

   Plasmid

   #122563

   Purpose

   Integration of QF2 sequence using the CRISPR/Cas9 system.

   Depositor

   Marnie Halpern

   Article

   Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

   Insert

   Gbait-hsp70-QF2-SV40pA
   

   Use

   Cloning

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. HP-Cas9hGem-Ade2-LEU2-LINEAR

    Plasmid

    #174839

    Purpose

    pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial and Yeast

    Promoter

    TDH3p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMT-halo-HA-p2a-neoR

    Plasmid

    #161977

    Purpose

    homologous donor template for endogenous tagging

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    GAlinker-HA-halo-HA-p2a-neoR
    

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMT-puroR-p2a-HA-halo

    Plasmid

    #161979

    Purpose

    homologous donor template for endogenous tagging

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    puroR-p2a-HA-halo-GSlinker
    

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM-T Easy-Gbait-hs-Cre

    Plasmid

    #122562

    Purpose

    Integration of Cre sequence using the CRISPR/Cas9 system.

    Depositor

    Marnie Halpern

    Article

    Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

    Insert

    Gbait-hsp70-Cre-SV40pA
    

    Use

    Cloning

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-sgIGR_dual-MTS-3x-Set I

    Plasmid

    #162764

    Purpose

    Separately expressing 3 unique sgIGR_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rab18b cds in pGem-T Easy

    Plasmid

    #80539

    Purpose

    Can be used for in situ hybridisation or other purposes

    Depositor

    Rob Parton

    Article

    Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

    Insert

    rab18b (rab18b Zebrafish)
    

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Rab18a cds in pGem-T Easy

    Plasmid

    #80538

    Purpose

    Can be used for in situ hybridisation or other purposes

    Depositor

    Rob Parton

    Article

    Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

    Insert

    rab18a (rab18a Zebrafish)
    

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMT-Pax7bait-T2a-Cherry-pA

    Plasmid

    #111154

    Purpose

    knock in bait for Axolotl Pax7 locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Proc Natl Acad Sci U S A. 2017 Nov 21;11

    Insert

    Pax7bait-T2a-Cherry-pA
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM-rat FceRI alpha

    Plasmid

    #8373

    Depositor

    Jean Kinet

    Article

    Kinet et al Biochemistry 1987 Jul 28;26(15):4605-10.

    Insert

    rat Fc epsilon RI alpha cDNA (Fcer1a Rat)
    

    Use

    Beta-gal

    Expression

    Bacterial

    Available Since

    June 7, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pGEMT-aid-p2a-GFP

    Plasmid

    #161976

    Purpose

    homologous donor template for endogenous tagging

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    GSlinker-aid-GFP
    

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMT-GFP-p2a-aid

    Plasmid

    #161975

    Purpose

    homologous donor template for endogenous tagging

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    GFP-aid-GSlinker
    

    Available Since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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