We narrowed to 32,048 results for: ACE
-
Plasmid#65991PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23107_GB
Plasmid#65999PurposeMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23116_GB
Plasmid#66003PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK760
Plasmid#192643PurposeExpresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpRDepositorInsertBBa_J23110-sfGFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23110Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSub-MBP-GFP(2M-IKQE)
Plasmid#185757PurposeBacterial expression of MBP-GFP(2M IKQE)DepositorInsertGFP(2M-IKQE)
UseTagsMBPExpressionBacterialMutationPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral-KOR-FRB
Plasmid#191496PurposeRapamycin-dependent opioid sensor for the kappa opioid receptor (component 1)DepositorInsertKOR-FRB
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMD19-PBBa_J23117-sgRNA-Spr
Plasmid#190793PurposeTemplate vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.DepositorInsertsgRNA (dummy)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBBa_J23117Available sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.(cyto).cpSFGFP.HaloTag
Plasmid#209662PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVTagsExpressionMutationPromoterGFAPAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Spy0469
Plasmid#159996PurposeExpresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cellsDepositorInsertSpy0469 (24-389)
UseTagsHis6-tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/plsA
Plasmid#73625PurposeProduces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tagDepositorInsertadenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYSD153
Plasmid#212929PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD141
Plasmid#212928PurposeEncodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.DepositorInsertmRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
BLING 1
Plasmid#171647PurposeExpresses bioluminescent glutamate indicator in mammalian cellsDepositorInsertBLING 1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC-SmBiT
Plasmid#207632PurposeA plasmid encoding photocaged SpyCatcher (pSC) fused to SmBiTDepositorInsertphotocaged SpyCatcher-SmBiT
UseTags6xHisExpressionBacterialMutationAmber stop codon at SC's critical lysinePromoterT7Available sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2_N_deltaNIDR
Plasmid#157868Purposeexpress MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDRDepositorInsertN (N SARS-CoV-2)
UseTagsMBPExpressionBacterialMutationdeltaNIDRPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMGE-T7
Plasmid#153021PurposeSingle-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-a (colE1 origin)
Plasmid#74091PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-M-Gal4
Plasmid#84554PurposeMESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivatorDepositorInsertRap-MESA-FRB-20-M-Gal4
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-4
Plasmid#50738PurposeExpresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-4 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
cacnb1b (rat) in pMT2 vector
Plasmid#107423PurposeExpresses Calcium channel beta1b auxillary subunit in mammalian cells.DepositorInsertcalcium channel beta1b auxillary subunit
UseTagsExpressionMammalianMutationPromoterAd MLPAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF4 CTF-FLAG
Plasmid#217697PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF4 (ADGRF4 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-384PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE1 CTF-FLAG
Plasmid#217689PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE1 (EMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-584PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
CTAG_mScarlet-I
Plasmid#136118PurposeFP for C-term fusion with CDS12DepositorInsertCTAG-mScarletI(noATG)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV-mEGFP(A206K)-paCaMKII(K42M)-ShadowG
Plasmid#167477PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(K42M)-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP(A206K)-paCaMKII(T286A)-ShadowG
Plasmid#167476PurposeExpresses paCaMKII in mammalian cells to measure structural change of paCaMKII.DepositorInsertmEGFP(A206K)-paCaMKII(T286A)-ShadowG
UseTagsShadowG and mEGFP(A206K)ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS4
Plasmid#55751PurposeProduces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.DepositorInsertKanR
UseTagsExpressionBacterialMutationResidues 1-59 of KanRPromoterT7-lacAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD-tdTEVDB_C-15A/G4C
Plasmid#177205PurposePermuted td intron with evolved initiation sequence for the expression of circular mRNADepositorInsertPermuted GFP with evolved initiation sequence for circular mRNA
UseTags6x HisExpressionBacterialMutationPermuted sfGFP after residue 52 & Changed Cyt…PromoteraraBADAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
U6-spTRE3G-CMV-mTagBFP2
Plasmid#102854PurposeA plasmid encoding TRE3G sgRNA (for SpCas9) and mTagBFPDepositorInsertsp-TRE3G-sgRNA; mTagBFP2
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTU425
Plasmid#178984PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)DepositorInsertTPR-CHAT H585A C627A
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR
Plasmid#100008PurposeExpresses Cas9-Nlux and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into genomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-NluxExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-1
Plasmid#50739PurposeExpresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-1 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD
Plasmid#197687Purposeplasmid for expressing the DNMT3bCDDepositorInsertH2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Human, Synthetic)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-HCA_sensor
Plasmid#127182PurposeEukaryotic expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
UseTagsHisTag and StrepTagExpressionMammalianMutationPromoterCMV-TetO2Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA GCNF
Plasmid#16336DepositorInsertGCNF (Nr6a1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAL1
Plasmid#197285PurposeShuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195DepositorInsertOrigin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZA-GFP-R5
Plasmid#106256PurposeConstitutively expresses GFP fused with silaffin-R5 silica affinity peptideDepositorInsertGreen fluorescent protein
UseTagsAdded silaffin R5 peptide to C terminus of GFP af…ExpressionBacterialMutationPromoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
FabF (KASII) pet28 N-terminal His Tag
Plasmid#75017PurposeExpresses the ketosynthase FabF/KASII from the E. coli fatty acid synthaseDepositorInsertFabF (fabF E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK671.306
Plasmid#192641PurposeExpresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmRDepositorInsertJ306 scRNA
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/prsA
Plasmid#73624PurposeProduces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tagDepositorInsertribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only