We narrowed to 8,330 results for: 11
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Plasmid#177804PurposeAn intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.DepositorInsertintron
ExpressionMammalianAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FH1-MGT#1-mVenus
Plasmid#164094PurposeFluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.DepositorInsertMGT#1-mVenus
UseLentiviral and Synthetic BiologyAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-fDIO-GRAB_eCB2.0
Plasmid#164610PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cellsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVMutationS383TPromoterEFSAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK0_057
Plasmid#123950PurposeEncodes the hAAVS1 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInsertBxBI LP on hAAVS1 (Hygro::mRuby)
ExpressionMammalianAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_062
Plasmid#123951PurposeEncodes the 2nd gen. Lenti Virus GFP dropout destination vector without insulator as a type 0 part to be used in the MTK systemDepositorInsertpHR DEST
ExpressionMammalianAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-mRuby2-roGFP1
Plasmid#107219PurposeMammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.DepositorInsertmRuby2-roGFP1
Tags4xCox8ExpressionMammalianPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCol1a1-frt (FVB)
Plasmid#63575PurposeTargeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.DepositorInsertCol1A-frt-hygro-pA
UseMouse Targeting; Frt/flpeExpressionBacterial and MammalianPromoterNoneAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDG04439
Plasmid#216365PurposexhNup133-Nb2t (+2Cys) Nanobody productionDepositorInsertxhNup133-Nb2t (+2Cys)
UseAffinity Reagent/ AntibodyTags14xHis-NEDD8ExpressionBacterialAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pN1-RnKIF5C(1-560)-Halo-Flag
Plasmid#188071PurposeMammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tagsDepositorAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha1
Plasmid#165862PurposeAlpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-Nr4a1 double gRNAs
Plasmid#162791PurposeMultiplex Guide RNAs to generate Nr4a1 knockout by CRISPRDepositorAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI
Plasmid#140202PurposeCRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.DepositorInsertmCherry
UseCRISPR and Synthetic Biology; Proof-of-concept te…MutationG174DPromoterPelcAAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PDF1::mCitrine-MBD
Plasmid#115222PurposeMicrotubule visualizationDepositorInsertMicrotubule binding domain
TagsmCitrineExpressionPlantAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TFAP4(E135A/S139A)
Plasmid#110157PurposeExpression of human TFAP4 (E135A/S139A) in mammalian cellsDepositorInsertTFAP4 (transcription factor AP-4) (TFAP4 Human)
TagsHAExpressionMammalianMutationE135A/S139APromoterCMVAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-AuroraB L154G/H250Y
Plasmid#108492Purposeexpression of EGFP-AuroraB Analog sensitive (L154G/H250Y)DepositorInsertAuroraB (AURKB Human)
TagsEGFPExpressionMammalianMutationAnalog sensitive L154G/H250YPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3-Flag-AuroraB KD (K106R)
Plasmid#108488Purposeexpression of Flag-AuroraB kinase death (K106R)DepositorInsertAuroraB (AURKB Human)
TagsFlagExpressionMammalianMutationKinase death K106APromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV Puro SCRIB P305L
Plasmid#88887PurposeSCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralMutationProline to Leucine mutation @ 305th amino acidPromoterMSCV promoterAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hSTARR-seq_SCP1 vector_blocking 3
Plasmid#99318PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorExpressionMammalianAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAL-p300-1000-1600
Plasmid#89101Purposemammalian expression of human p300 amino acids 1000-1600 with N-terminal GAL4 DNA binding domainDepositorAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only