We narrowed to 11,615 results for: Vars

1. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

   Plasmid

   #120567

   Purpose

   Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, Y40A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

   Plasmid

   #120566

   Purpose

   Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, E37A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpG-P2A-EGFP (RTW4177)

   Plasmid

   #139988

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-FGFR3c-V5/HIS

   Plasmid

   #201108

   Purpose

   expression of human FGFR3 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

   Plasmid

   #120561

   Purpose

   Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   KRAS4B (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pETM33_Nsp5_Mpro

   Plasmid

   #156475

   Purpose

   Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro

   Plasmid

   #120559

   Purpose

   Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   HRAS (HRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCKC775

   Plasmid

   #226626

   Purpose

   piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
   EF1a promoter, sfGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   7xTCF-LEF and EF1a

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCKC764

   Plasmid

   #226625

   Purpose

   piggyBAC pCAG-hCas9-T2A-Unbiased3

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, S. pyogenes, Chicken)
   EF1a promoter, blasticidin resistance
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV and EF1a

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Promoter

    4xHRE_YB TATA and EF1a

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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