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pnEK-NvS_M

Plasmid

#146926

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Article

Diebold et al J Struct Biol. 2011 Aug;175(2):178-88. d

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X2

Plasmid

#176152

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-X3

Plasmid

#176153

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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BB2_AB

Plasmid

#98534

Purpose

empty BB2 for transcription unit cloning

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Bxbi GFP MCP Reporter

Plasmid

#78225

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Available Since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-HA no-pA

Plasmid

#82593

Purpose

3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

HA tag (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pTac-A control-luci

Plasmid

#65939

Purpose

No-Aptamer Control for Theophylline Riboswitch A with luciferase gene

Depositor

Justin Gallivan

Article

Mishler et al Nucleic Acids Res. 2014 Jun;42(10):6753-

Insert

luciferase

Expression

Bacterial

Available Since

Sept. 3, 2015

Availability

Academic Institutions and Nonprofits only
MCS_BsmBI

Plasmid

#241170

Purpose

Middle entry vector for mini-Golden subcloning platform; MCS; BsmBI

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

MCS; BsmBI

Use

Mini-golden

Mutation

TCGC linker

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3b_029_mScarlet-I

Plasmid

#235882

Purpose

mScarlet-I N-tag

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

mScarlet-I N-tag

Use

Synthetic Biology

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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CROP-seq-puro-v2-no-scaffold

Plasmid

#228521

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

Depositor

Jellert Gaublomme

Article

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Inserts

Use

Lentiviral

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pVEntry

Plasmid

#207674

Purpose

Entry vector for Trio vector system, with vanillate-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPEntry

Plasmid

#207673

Purpose

Entry vector for Trio vector system, with DAPG-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCEntry

Plasmid

#207672

Purpose

Entry vector for Trio vector system, with cumate-inducible promoter

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSEVA43-GG

Plasmid

#208014

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPomyces-AsCas12j-2

Plasmid

#191655

Purpose

All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

Depositor

Fong Tian Wong

Article

Tan et al Biomolecules. 2024 Apr 16;14(4):486. doi

Insert

AsCas12j-2

Expression

Bacterial

Promoter

rpsL(XC)-BbsI

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pETTEV-SENP8

Plasmid

#196361

Purpose

protein expression of the His-tagged full length protein

Depositor

Stefan Knapp

Insert

SENP8/NEDP1

Tags

His6-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRK010

Plasmid

#69874

Purpose

expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

D156C (XXL)

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Puro

Plasmid

#167895

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EYFP

Plasmid

#167890

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_EBFP2

Plasmid

#167889

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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