We narrowed to 12,310 results for: LEA
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Plasmid#19845DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMKL1 S449A/T450A/S454A (MRTFA Human)
Tags3xFLAGExpressionMammalianMutationchanged Serine 449, Threonine 450 and Serine 454 …Available sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisplay-FRISZ
Plasmid#176887PurposeFar-red fluorescent zinc indicator FRISZ in pDisplayDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRISZ
TagspDisplay backbone elements for export and membran…ExpressionMammalianPromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
3XHalo_GFP_TRF1#1
Plasmid#164644Purposetag TRF1 with GFP and HaloDepositorAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX-2A-2A
Plasmid#74670Purposebased on pCX-C1 with F2A1/F2A2 insertedDepositorTypeEmpty backboneExpressionMammalianPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEMS1228
Plasmid#29114PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle246 (VIM Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSAvailable sinceNov. 4, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS-M71-IRES-CreFNF
Plasmid#15509DepositorPublicationInsertM71 targeting vector with IRES CreFNF
UseHigh copy numberAvailable sinceAug. 24, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
V5-BS2-NES (Cytosol)
Plasmid#220341PurposeMammalian expression vector BS2 in cytoplasmDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBS2
TagsNES (Nuclear exclusion sequence) and V5 tagExpressionMammalianPromoterCMVAvailable sinceJune 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGGS-N-TEV-Twin-Strep
Plasmid#202526PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cellDepositorTypeEmpty backboneTagsTEV cleavable site and Twin-strep-tagExpressionMammalianPromoterCAGAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV_eA3A-nCas9
Plasmid#163535PurposeMammalian CG-to-GC base editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserteA3A-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)Available sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCK005_U6-Sp-sgRNA_UP-TdTomato_WPRE
Plasmid#85453PurposeLentiviral CRISPR system backbone bearing BsmBI site for S. pyogenes new guide RNAs and TdTomato.DepositorInsertU6-sgRNA-Backbone-UP-TdTomato
UseCRISPR and LentiviralTagsNLSExpressionMammalianMutationdeletion of AA123-365 in tdTomato (please see dep…Available sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDN43
Plasmid#61346PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry
TagsGS linker-AsLov2-biNLS11 and PKIt NESExpressionMammalianPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRTCLCrVA.008
Plasmid#22841DepositorInsertA DNA (with MCS)
UseCotton vigs vectorExpressionBacterialMutationCoat Protein (AR1 gene) of Cotton Leaf Crumple Vi…Available sinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
ND4.2-Right DdCBE-G1397-C- T1413I
Plasmid#179685PurposeExpression of base editor in mammalian cellsDepositorInsertSOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR
ExpressionMammalianMutationT1413IAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam mse LaminA
Plasmid#182674PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.DepositorInsertLamin A (Lmna Mouse)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianPromoterHSPAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)
Plasmid#181745Purposedual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1DepositorInserthuman/zebrafish codon optimized SpCas9
UseIn vitro transcription; t7 promoterTagsNLS(SV40)-3xFLAGExpressionBacterialPromoterT7Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TfR-moxGFP-DHFR
Plasmid#173012PurposeFor light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter. Fused to oxidation resistant version of GFP (moxGFP).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTfR-moxGFP-DHFR
TagsDHFR and moxGFPExpressionMammalianPromoterCMVAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA
Plasmid#129708PurposeExpress cre-dependent KASH-EGFP and scramble shRNADepositorInsertKASH-EGFP
UseAAVTagsEGFPExpressionMammalianPromoterU6Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9-D10A
Plasmid#61477Purposebased on pDe-CAS9, encodes Cas9 D10A nickase variantDepositorInsertCas9-D10A
UseCRISPRExpressionPlantMutationcatalytic Asp10 changed to AlaPromoterPcUbi4-2Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemExpressionMammalianMutationY64L (mutRFP)Available sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPE-1m
Plasmid#13540DepositorInsertFLIP-E A207W
TagsECFP and VenusExpressionBacterialMutationA207W in binding proteinAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only