We narrowed to 13,452 results for: Mpl;

1. pHAGE-RNF8-C403S

   Plasmid

   #221703

   Purpose

   Lentiviral expression of human RNF8 (C403S) in mammalian cells

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   RNF8 (RNF8 Human)
   

   Use

   Lentiviral

   Mutation

   C403S

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE-RNF8-S157A

   Plasmid

   #221704

   Purpose

   Lentiviral expression of human RNF8 (S157A) in mammalian cells

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   RNF8 (RNF8 Human)
   

   Use

   Lentiviral

   Mutation

   S157A

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-FLAG-MAD2-RQ

   Plasmid

   #221691

   Purpose

   Mammalian expression of FLAG-tagged human MAD2 (R133E/Q134A)

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   MAD2
   

   Expression

   Mammalian

   Mutation

   R133E, Q134A

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-FLAG-MAD2

   Plasmid

   #221690

   Purpose

   Mammalian expression of FLAG-tagged human MAD2

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   MAD2
   

   Expression

   Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-FLAG-p31(comet)

   Plasmid

   #221692

   Purpose

   Mammalian expression of FLAG-tagged human p31(comet)

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   MAD2BP1
   

   Expression

   Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-FLAG-p31(comet)-QF

   Plasmid

   #221693

   Purpose

   Mammalian expression of FLAG-tagged human p31(comet) (Q83A/F191A)

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   MAD2BP1
   

   Expression

   Mammalian

   Mutation

   Q83A, F191A

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-FLAG-RNF8

   Plasmid

   #221695

   Purpose

   Mammalian expression of FLAG-tagged human RNF8

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   RNF8 (RNF8 Human)
   

   Expression

   Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Hygro-P2A-HA-FKBP-WAPL

   Plasmid

   #217666

   Purpose

   Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   WapI (WAPL Human)
   

   Tags

   dTag (FKBP36V protein)

   Expression

   Bacterial and Mammalian

   Promoter

   Lac

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-RepoMan-RATA

   Plasmid

   #184270

   Purpose

   Expresses shRNA resistant GFP-RepoMan RATA Mutant

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   cell division cycle associated 2 (CDCA2 Human)
   

   Tags

   3xHA and GFP

   Expression

   Mammalian

   Mutation

   V383A, F395A

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-RepoMan-T394A

    Plasmid

    #204994

    Purpose

    Expresses shRNA resistant GFP-RepoMan T394A Mutant

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    cell devision cycle associated 2 (CDCA2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed Threonine 394 to Alanin

    Promoter

    CMV

    Available Since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PMP34-mApple-NFAST(low)

    Plasmid

    #214418

    Purpose

    Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N (SLC25A17 )
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOPINOH11-A10

    Plasmid

    #184278

    Purpose

    Expresses nanobody H11-A10 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-A10
    

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOPINO_H11-B5

    Plasmid

    #184279

    Purpose

    Expresses nanobody H11-B5 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-B5
    

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOPINO_11-H6

    Plasmid

    #184280

    Purpose

    Expresses nanobody H11-H6 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-H6
    

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOPINH11-H4 Q98R_H100E

    Plasmid

    #184281

    Purpose

    Expresses nanobody H11-H4 Q98R_H100E in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11-H4 Q98R_H100E
    

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Kv2.1 K+ channel scFv [D4/154]

    Plasmid

    #206787

    Purpose

    Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma D4/154 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Kv2.1 K+ channel recombinant scFV
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(TAL1(11))-PGKpuro2ABFP-W

    Plasmid

    #208545

    Purpose

    Lentiviral vector expressing gRNA targeting human TAL1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    TAL1(11) (TAL1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(TAL1(12))-PGKpuro2ABFP-W

    Plasmid

    #208546

    Purpose

    Lentiviral vector expressing gRNA targeting human TAL1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    TAL1(12) (TAL1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(MRPS26(15))-PGKpuro2ABFP-W

    Plasmid

    #200499

    Purpose

    Lentiviral vector expressing gRNA targeting human MRPS26

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    MRPS26(15) (MRPS26 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2.2-h7SKgRNA5(AAVS1)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #200502

    Purpose

    Lentiviral vector expressing gRNA targeting human AAVS1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    AAVS1 (AAVS1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKLV2-U6gRNA5(MRPS26(12))-PGKpuro2ABFP-W

    Plasmid

    #200498

    Purpose

    Lentiviral vector expressing gRNA targeting human MRPS26

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    MRPS26(12) (MRPS26 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-W

    Plasmid

    #200465

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA2

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA2(46) (SMARCA2 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV2-U6gRNA5(NDUFA1(12))-PGKpuro2ABFP-W

    Plasmid

    #200495

    Purpose

    Lentiviral vector expressing gRNA targeting human NDUFA1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    NDUFA1(12) (NDUFA1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKLV2-U6gRNA5(MRPL21(16))-PGKpuro2ABFP-W

    Plasmid

    #200497

    Purpose

    Lentiviral vector expressing gRNA targeting human MRPL21

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    MRPL21(16) (MRPL21 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKLV2-U6gRNA5(NDUFA1(11))-PGKpuro2ABFP-W

    Plasmid

    #200494

    Purpose

    Lentiviral vector expressing gRNA targeting human NDUFA1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    NDUFA1(11) (NDUFA1 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKLV2-U6gRNA5(MRPL21(14))-PGKpuro2ABFP-W

    Plasmid

    #200496

    Purpose

    Lentiviral vector expressing gRNA targeting human MRPL21

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    MRPL21(14) (MRPL21 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CCM2_d410-444_eGFP

    Plasmid

    #208877

    Purpose

    Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids deletion : 410-444

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

    Plasmid

    #207136

    Purpose

    DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. qTAG-N-Blast-V5-LMNB1

    Plasmid

    #207779

    Purpose

    Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    LMNB1 Homology Arms flanking a Blast-V5 Cassette (LMNB1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. qTAG-C-Solo-mScarlet-H3C2

    Plasmid

    #207781

    Purpose

    Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. qTAG-C-mNeon-Blast-H3C2

    Plasmid

    #207782

    Purpose

    Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mNeon-Blast Cassette (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. An HA CAAX pMT2

    Plasmid

    #206116

    Purpose

    expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. qTAG-N-Blast-moxGFP-TUBA1B

    Plasmid

    #207766

    Purpose

    Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Blast-moxGFP Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. An CAAX pMT2

    Plasmid

    #206118

    Purpose

    expression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motif

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Expression

    Mammalian

    Promoter

    Ad MLP/TPL/SV40

    Available Since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TDP-43 _CTD_6F→A

    Plasmid

    #194269

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→A

    Depositor

    Nicolas Fawzi

    Article

    Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Insert

    TDP-43 (TARDBP Human, Synthetic)
    

    Tags

    6XHistag

    Expression

    Bacterial

    Mutation

    F276A, F283A, F289A, F367A, F397A, F401A

    Promoter

    T7

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TDP-43 _CTD_5M→A_CR

    Plasmid

    #194263

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5M→A_CR

    Depositor

    Nicolas Fawzi

    Article

    Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Insert

    TDP-43 (TARDBP Human, Synthetic)
    

    Tags

    6XHistag

    Expression

    Bacterial

    Mutation

    M322A, M323A, M336A, M337A, M339A mutations in TD…

    Promoter

    T7

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TDP-43 _CTD_5M→A_IDR

    Plasmid

    #194264

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to Alanine

    Depositor

    Nicolas Fawzi

    Article

    Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Insert

    TDP-43 (TARDBP Human, Synthetic)
    

    Tags

    6XHistag

    Expression

    Bacterial

    Mutation

    M307A, M311A, M359A, M405A, M414A mutations in TD…

    Promoter

    T7

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TDP-43 _CTD_5M→L_IDR

    Plasmid

    #194265

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to leucine

    Depositor

    Nicolas Fawzi

    Article

    Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    6XHistag

    Expression

    Bacterial

    Mutation

    M307L, M311L, M359L, M405L, M414L mutations in TD…

    Promoter

    T7

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TDP-43 _CTD_5M→Y_IDR

    Plasmid

    #194266

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Methionines to tyrosine

    Depositor

    Nicolas Fawzi

    Article

    Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Insert

    TDP-43 (TARDBP Human, Synthetic)
    

    Tags

    6XHistag

    Expression

    Bacterial

    Mutation

    M307Y, M311Y, M359Y, M405Y, M414Y mutations in TD…

    Promoter

    T7

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. TDP-43 _CTD_5A→M_IDR

    Plasmid

    #194267

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with 5 Alanines to methionine

    Depositor

    Nicolas Fawzi

    Article

    Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Insert

    TDP-43 (TARDBP Human, Synthetic)
    

    Tags

    6XHistag

    Expression

    Bacterial

    Mutation

    A297M, A367M, A381M, A388M, A391M mutations in TD…

    Promoter

    T7

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. GFP Cav2.2 D122A pcDNA3

    Plasmid

    #206068

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. 1BR9-AtWUSSTOP-Δα3

    Plasmid

    #202056

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
    

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    third helix of homeodomain deleted (AA82-102)

    Promoter

    T7 Promoter

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pUC19-π-EB1_IRES-mCherry

    Plasmid

    #197427

    Purpose

    Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GCN4 leucine zipper, LOV2, Zdk1, and mCherry

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pUC19-π-EB1_EF1α-mCherry

    Plasmid

    #197429

    Purpose

    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GCN4 leucine zipper, LOV2, Zdk1, and mCherry

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUC19-π-EB1_EF1α-EGFP

    Plasmid

    #197430

    Purpose

    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    EGFP, GCN4 leucine zipper, LOV2, and Zdk1

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid

    #201024

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pGBT9-FTS

    Plasmid

    #198526

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FTS fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FTS (AKTIP Human)
    

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pGBT9-FHIP-L

    Plasmid

    #198528

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-FHIP-L fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    FHIP-L (FHIP1A Human)
    

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pGADT7-δ

    Plasmid

    #197259

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-3 δ (AP3D1 Human)
    

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Val 549 Ile substitution; silent substitutions in…

    Promoter

    ADH1

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBridge-tyrosinase.σ3A

    Plasmid

    #197513

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-3 σ3A
    

    Tags

    GAL4-DNA binding domain fragment, HA tag, and nuc…

    Expression

    Yeast

    Mutation

    silent substitution in codon 2 of tyrosinase tail…

    Promoter

    ADH1 and MET25

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge