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Cs2+Etv4(pea3)

Plasmid

#49093

Purpose

expression of Etv4

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pST_AfrLEA3m (pBS0911)

Plasmid

#185285

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pST_PvLEA4_repeats_k3_26 (pBS0762)

Plasmid

#185244

Purpose

For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1899)

Plasmid

#170143

Purpose

Expresses residues 1-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1900-2997

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (1-1547)

Plasmid

#170144

Purpose

Expresses residues 1-1547 of CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

C-terminal truncation of residues 1548-2997

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (K999R)

Plasmid

#170142

Purpose

Expresses CHD7 (K999R) in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Mutation

K999R

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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GST-rhodopsin-CTS

Plasmid

#86071

Purpose

A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Rhodopsin (RHO Bovine)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 309-348 of Rhodopsin

Promoter

tac

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T-2 MED8

Plasmid

#15436

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED8 (MED8 Human)

Tags

GST

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
CFF-EGFP

Plasmid

#86061

Purpose

A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

contains luminal and transmembrane domains of CD8…

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pST_SERF2_HUMAN (pBS0751)

Plasmid

#185239

Purpose

For the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

SERF2_HUMAN

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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CBP-Bromo-HAT-CH3-sp65

Plasmid

#21026

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT+CH3 domains aa 1098-1877; E1239G mutati…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pST_MAHS_RAMVA (pBS0310)

Plasmid

#185169

Purpose

For the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

MAHS_RAMVA

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX17WHC

Plasmid

#118839

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17WHC (Sox17 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

WHC: L46W, V53H, E57C

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA SOX17 -91

Plasmid

#50923

Purpose

U6 driven sgRNA targeting Sox17 -91 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17 -91 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pST_DHN_AtCor47 (pBS0784)

Plasmid

#185254

Purpose

For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_AtCor47

Expression

Mammalian

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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mPapaya-Keratin14-N-18

Plasmid

#56346

Purpose

Localization: Cytokeratin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Keratin14 (KRT14 Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pCGN-HCF-1-N450-1011

Plasmid

#92323

Purpose

N-term 450-1011 of HCF-1 protein

Depositor

Winship Herr

Article

Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

Insert

HCF-1 (HCFC1 Human)

Tags

HA

Expression

Mammalian

Mutation

AA 450-1011

Promoter

CMV

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2238

Plasmid

#36689

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD7-TALEN_Left (Chd7 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2239

Plasmid

#36690

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CHD7-TALEN_Right (Chd7 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA GAL4 CREB 1-341 M1

Plasmid

#22973

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Apr . 11(4):1101-8.

Insert

CREB (Creb1 Rat, GAL4-fusion)

Tags

GAL4 DNA-binding domain

Expression

Mammalian

Mutation

Changed Serine 133 to Alanine of CREB

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only

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