We narrowed to 73,573 results for: LAS

1. pCAG-OSF-VPS34

   Plasmid

   #99327

   Purpose

   Component for mammalian expression of recombinant PI3KC3-C1 complex

   Depositor

   James Hurley

   Article

   Baskaran et al Elife. 2014 Dec 9;3. doi: 10.7554/eLife.

   Insert

   VPS34 (PIK3C3 Human)
   

   Use

   Tags

   Strep-Strep-Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

2. TR-Csy4-R115A/R119A

   Plasmid

   #80696

   Purpose

   Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

   Depositor

   Aravind Asokan

   Article

   Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

   Insert

   Csy4-R115A/R119A
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Arg115 and Arg119 both mutated to Ala

   Promoter

   CBA

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEN716 - pCAGGS-eGFP-3Xnls-bpA

   Plasmid

   #156435

   Purpose

   For transient expression of nuclear eGFP in mammalian cells

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGal4BD-CRY2PHR

   Plasmid

   #28244

   Purpose

   Expresses Gal4 DNA Binding Domain fused to CRY2PHR (aa1-498) for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   cry2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-PKCb1-mEGFP-N1

   Plasmid

   #112265

   Purpose

   ITRACKbeta/IDOCKSbeta Donor construct

   Depositor

   Ryohei Yasuda

   Article

   Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

   Insert

   protein kinase c beta (Prkcb Mouse)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac1 PIK3R1

   Plasmid

   #17743

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

   Insert

   PIK3R1 (PIK3R1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pDR-GFPuniv

   Plasmid

   #46085

   Purpose

   In vivo recombination assay fluorescent reporter

   Depositor

   Raymond Monnat

   Article

   Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

   Insert

   DR-GFPuniv reporter
   

   Use

   Recombination reporter plasmid

   Tags

   Expression

   Mammalian

   Mutation

   E5G and K163R mutations in EGFP relative to wild-…

   Promoter

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-PDZD8HA_sh_resistant

   Plasmid

   #105006

   Purpose

   Mammalian Expression of PDZD8, sh resistant

   Depositor

   Franck Polleux

   Article

   Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

   Insert

   PDZD8 (Pdzd8 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   The sequence recognized by shPDZD8 (TRCN000043105…

   Promoter

   CAG

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM002-sgRNA-lenti-ZeoR

   Plasmid

   #200060

   Purpose

   Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

   Depositor

   Britt Glaunsinger

   Article

   McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

   Insert

   Bleomycin resistance, mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLGCV

    Plasmid

    #49862

    Purpose

    Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).

    Depositor

    Jonathan Pettitt, Anne Glover

    Article

    Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118

    Insert

    firefly luciferase luc+ fused to GFP (S65C)
    

    Use

    Tags

    GFP (S65C)

    Expression

    Worm

    Mutation

    Promoter

    sur-5

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-TDsmURFP-RBS-HO-1

    Plasmid

    #80342

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

    Depositor

    Roger Tsien, Erik Rodriguez

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Use

    Tags

    6 Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEN_TTmiRc2

    Plasmid

    #25752

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. RCAS-myrAkt

    Plasmid

    #11547

    Depositor

    Sandra Orsulic

    Article

    Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

    Insert

    Protein Kinase B (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag and myr signal

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-BE4-SsAPOBEC3B R54Q

    Plasmid

    #138344

    Purpose

    Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-SsAPOBEC3B R54Q
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMPMA3dPlac-PssaG-GFPLVA

    Plasmid

    #23343

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology (Reading). 2010 Apr;156(Pt

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA5-FRT/TO-LacR-TopBP1

    Plasmid

    #31313

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    FLAG and LacR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pEN_TmiR_Luc-A

    Plasmid

    #25760

    Purpose

    Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. CAGhP14

    Plasmid

    #84362

    Purpose

    express FLAG-HA tagged human PRDM14

    Depositor

    Danny Reinberg

    Article

    Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

    Insert

    PRDM14 (PRDM14 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    silent mutations at coding silent 1068CtoT, 1629T…

    Promoter

    CAG

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

19. KBB364

    Plasmid

    #185083

    Purpose

    RFA1 fused to GFP under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK1221

    Plasmid

    #84220

    Purpose

    Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, mCherry

    Depositor

    Martin Kampmann, Jonathan Weissman

    Article

    Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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