We narrowed to 75,913 results for: tra

1. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-PD-H166Q

   Plasmid

   #110179

   Purpose

   Expresses propanediol dehydratase mutant H166Q in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Propanediol Dehydratase
   

   Use

   Tags

   His6 and S-tag

   Expression

   Bacterial

   Mutation

   H166Q mutant

   Promoter

   T7

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET29b-PD-E440Q

   Plasmid

   #110178

   Purpose

   Expresses propanediol dehydratase mutant E440Q in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Propanediol Dehydratase
   

   Use

   Tags

   His6 and S-tag

   Expression

   Bacterial

   Mutation

   E440Q mutant

   Promoter

   T7

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET29b-PD-C438S

   Plasmid

   #110177

   Purpose

   Expresses propanediol dehydratase mutant C438S in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Propanediol Dehydratase
   

   Use

   Tags

   His6 and S-tag

   Expression

   Bacterial

   Mutation

   C438S mutant

   Promoter

   T7

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET29b-PD-G817A

   Plasmid

   #110174

   Purpose

   Expresses propanediol dehydratase mutant G817A in E. coli BL21(DE3)

   Depositor

   Emily Balskus

   Article

   Levin et al Science. 2017 Feb 10;355(6325). pii: 355

   Insert

   Propanediol Dehydratase
   

   Use

   Tags

   His6 and S-tag

   Expression

   Bacterial

   Mutation

   G817A mutant

   Promoter

   T7

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

   Plasmid

   #107739

   Purpose

   mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   NLS::cGAL(DBD)::gp41-1-N-intein(C1A)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   change the first cysteine of gp41-1 N-intein to a…

   Promoter

   C. elegans myo-2 promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AY23_pgRNA.R5.2

   Plasmid

   #100295

   Purpose

   CCR5-targeting gRNA expression plasmid

   Depositor

   Manuel Goncalves

   Article

   Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

   Insert

   gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6 Polymerase III snRNA

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CFFΔC-EGFP

   Plasmid

   #86059

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains CD8a luminal and transmembrane domains a…

   Promoter

   CMV

   Available since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CFF-Myc

   Plasmid

   #86062

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus Myc-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   contains luminal and transmembrane domains of CD8…

   Promoter

   CMV

   Available since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CFFΔC-Myc

    Plasmid

    #86060

    Purpose

    A mammalian expression plasmid encoding fibrocystin mimentic clone CFFΔC (CD8a luminal domain, fibrocystin transmembrane and ciliary targeting signal domains) with C-terminus Myc-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Fibrocystin (Pkhd1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    contains CD8a luminal and transmembrane domains a…

    Promoter

    CMV

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

    Plasmid

    #61796

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Ywhab 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

    Plasmid

    #61793

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Xbp1 3'UTR with miR-200c MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

    Plasmid

    #61791

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad5 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

    Plasmid

    #61790

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Map3k1 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

15. psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

    Plasmid

    #61789

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    both miR-200c MRE-2 and MRE-3 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

16. psiCHECK-2-Crtap-3'UTR-MRE-1 MUT

    Plasmid

    #61786

    Purpose

    Luciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutated

    Depositor

    Judy Lieberman

    Article

    Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

    Insert

    mouse Crtap 3'UTR with miR-200c MRE-1 mutated
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    miR-200c MRE-1 mutated

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pJ02B2Rm_EF

    Plasmid

    #66060

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJ02B2Rm_AE

    Plasmid

    #66065

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJ02B2Rm_EF

    Plasmid

    #66066

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJ02B2Gm_AE

    Plasmid

    #66057

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge