We narrowed to 4,454 results for: TIL

1. hCathepsin L

   Plasmid

   #11250

   Purpose

   Mammalian expression of human cathepsin L

   Depositor

   Hyeryun Choe

   Article

   Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

   Has Service

   Cloning Grade DNA

   Insert

   cathepsin L (CTSL Human)
   

   Use

   Tags

   C9 (GTETSQVAPA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pDisCoTune

   Plasmid

   #173713

   Purpose

   Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

   Depositor

   Morten Norholm

   Article

   Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

   Inserts

   ERV1 (ERV1 Budding Yeast)
   PDI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFP

   Plasmid

   #176015

   Purpose

   Mammalian expression of EGFP. Parton lab clone DFS

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ULK2

   Plasmid

   #97075

   Purpose

   Expresses ULK2 in mammalian cells

   Depositor

   Pasquale Vito

   Article

   Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

   Insert

   ULK2 (ULK2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ-CBE-Cas9n-Act3.0

   Plasmid

   #178955

   Purpose

   It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP1

   Plasmid

   #79873

   Purpose

   Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

   Depositor

   Jason Peters, Carol Gross, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   xylA

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLM1110_LEU2

   Plasmid

   #168460

   Purpose

   Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

   Depositor

   Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Insert

   mRFP1
   

   Use

   Synthetic Biology; Yeast assembly vector for big-…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-Dual-iCas9-CMV

   Plasmid

   #167500

   Purpose

   Expression of dual inducible Cas9 with CMV promoter

   Depositor

   Jake Liang

   Article

   Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   Cas9
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK170.AAV-TRE-Cre-WPRE (Supernova)

   Plasmid

   #85040

   Purpose

   For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nlsCre-WPRE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Rab11a-7

    Plasmid

    #56444

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA3-Delta(Pro-MP)ADAM10-HA

    Plasmid

    #65107

    Purpose

    Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

    Insert

    Delta(Pro-MP)ADAM10 (ADAM10 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted AA 19-455

    Promoter

    CMV

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

12. GFP-CA-mDia1

    Plasmid

    #45583

    Depositor

    Ronen Zaidel-Bar, Klaus Hahn

    Article

    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

    Insert

    Constitutively active mDia1 (CA-mDia1) (Diaph1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Insert mDia1 contains mutations V161D and N165D i…

    Promoter

    CMV

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCDH-EF1a-eFFly-eGFP

    Plasmid

    #104834

    Purpose

    eFFly luciferase for invivo Imaging, eGFP for flourescent detection

    Depositor

    Irmela Jeremias

    Article

    Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

    Inserts

    eFFly
    eGFP
    

    Use

    Lentiviral and Luciferase; Egfp

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIRCgp41-1

    Plasmid

    #74227

    Purpose

    Vector for SICLOPPS-mediated protein circularization using the gp41-1 intein.

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Waldhauer et al Mol Biosyst. 2015 Nov 10;11(12):3231-43.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    gp41-1 C-intein and gp41-1 N-intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

    Plasmid

    #86674

    Purpose

    Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

    Depositor

    Nicolas Manel

    Article

    Raab et al Science. 2016 Apr 15;352(6283):359-62. d

    Insert

    GFP-FLAG-cGAS E225A/D227A
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_hSyn_WiChR_TS_mScarlet_ER

    Plasmid

    #205997

    Purpose

    High efficient, K+ selective channelrhodopsin for the inhibition of neurons

    Depositor

    Johannes Vierock, Peter Hegemann

    Article

    Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

    Has Service

    AAV9

    Insert

    Wobblia Inhibitory Channelrhodopsin
    

    Use

    AAV

    Tags

    TS, mScarlet, ER

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV_hSyn1-SIO-stGtACR2-FusionRed

    Plasmid

    #105677

    Purpose

    Expresses a soma-targeted GtACR2 in Cre-expressing neurons

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn1

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CKIIa-stGtACR2-FusionRed

    Plasmid

    #105669

    Purpose

    Expresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoter

    Depositor

    Ofer Yizhar

    Article

    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1

    Has Service

    AAV Retrograde and AAV1

    Insert

    stGtACR2
    

    Use

    AAV

    Tags

    FusionRed and Kv2.1

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. ROS lux sensor

    Plasmid

    #199791

    Purpose

    Synthetic gene circuit

    Depositor

    Timothy Lu

    Article

    Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

    Insert

    ROS lux Sensor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TccCas13a Twin SUMO

    Plasmid

    #199754

    Purpose

    Expresses codon optimized thermostabile TccCas13a in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    TccCas13a
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    LacI

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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