We narrowed to 7,747 results for: Trac;

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Sema3f(S,-)-Fc-His

Plasmid

#72150

Purpose

Expresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains a 93 nt deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3f(L,-)-AP-His

Plasmid

#72022

Purpose

Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains a 93 nt deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3f(S,-)-AP-His

Plasmid

#72024

Purpose

Expresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains a 93 nt deletion in exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3f(S, +)-Fc-His

Plasmid

#72149

Purpose

Expresses the Sema3F protein (truncated at cleavage site P1; ie, short and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3b(S)-Fc-His

Plasmid

#72140

Purpose

Expresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3b (Sema3b Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3b(S)-AP-His

Plasmid

#72014

Purpose

Expresses the Sema3B protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3b (Sema3b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET30Xa-SBL1(160E,NoCys)

Plasmid

#66875

Purpose

Expresses a cysteine-free version of pET30Xa-SBL1(160E) (4Cys to Ser) with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 (S160E) with all Cys to Ser mutations

Tags

Histag

Expression

Bacterial

Mutation

S160E; changed 4 (all) cysteines to serine

Promoter

T7lac

Available Since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1

Plasmid

#42185

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

G protein Gamma 3(F70A, F71A) (Gng3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

F70A, F71A

Promoter

cmv

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1

Plasmid

#42188

Depositor

Narasimhan Gautam

Article

O'Neill et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

Insert

Gamma 3(K68A,K69A) (Gng3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

K68A,K69A

Promoter

cmv

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 L555A

Plasmid

#19851

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L555A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 555 to Alanine

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only
pAV10.F3.loxP

Plasmid

#63200

Purpose

pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Availability

Academic Institutions and Nonprofits only

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pAV10.HO5.loxP

Plasmid

#63206

Purpose

pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8f

Plasmid

#169249

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8f

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eGFP-ALFA

Plasmid

#242764

Purpose

AAV transfer plasmid expressing eGFP-ALFA under a CAG promoter.

Depositor

Andrew Payne

Article

Park et al bioRxiv 2025.09.26.678648

Insert

EGFP

Use

AAV

Tags

ALFA

Promoter

CAG

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-BACE-HA

Plasmid

#232353

Purpose

Beta-Secretase to be expressed (1:1) with either ATLAS protein.

Depositor

Don Arnold

Article

Rivera et al Nat Methods. 2025 May;22(5):1101-1111. d

Has Service

AAV8

Insert

BACE (BACE1 Human)

Use

AAV

Tags

HA

Promoter

hSyn

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphasS-RLuc8

Plasmid

#140980

Purpose

Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphasS-RLuc8

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-mCh-Cry2WT

Plasmid

#101223

Purpose

FUS(1-214) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta3

Plasmid

#140988

Purpose

Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GBeta3 (GNB3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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